Recombinant Mouse DNA-directed RNA polymerase I subunit RPA34 (Cd3eap)

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Cat. No.
BT1172736
Source
Yeast
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1172736
Source
E.coli
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1172736
Source
E.coli
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1172736
Source
Baculovirus
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1172736
Source
Mammalian cell
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Purity
>85% (SDS-PAGE)
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Description

Molecular Overview of Cd3eap/RPA34

Cd3eap (CD3e molecule-associated protein), also termed RPA34 or PAF49, is a subunit of RNA polymerase I (Pol I) responsible for transcribing rRNA genes. Key attributes include:

PropertyDetail
Gene ID (Mouse)70333
Protein AliasesCAST, ASE-1, PAF49, DNA-directed RNA polymerase I subunit RPA34
Molecular Weight51 kDa (predicted: 46.2 kDa due to post-translational modifications)
Subcellular LocalizationNucleolus
Key DomainsLysine-rich C-terminal domain influencing nucleolar targeting

Recombinant Protein Production

Recombinant mouse Cd3eap is typically expressed in Escherichia coli for research applications. Commercial variants include:

Role in rRNA Transcription

  • Transcription Elongation: Cd3eap/RPA34 forms a heterodimer with RPA49 (PAF53 in humans) to enhance Pol I processivity. Deletion results in reduced rRNA production and defective ribosome biogenesis .

  • Nucleolar Structure:

    • In vivo studies show that Cd3eap knockout disrupts nucleolar morphology, increasing nucleolar volume by ~30% in yeast models .

    • Miller spread analyses reveal reduced Pol I clustering on rRNA genes in RPA34-deficient cells, impairing transcription efficiency .

Evolutionary Conservation

  • Functional complementation assays demonstrate cross-species compatibility:

    • Human CAST (Cd3eap orthologue) restores Pol I activity in S. cerevisiae when co-expressed with human PAF53 .

    • Schizosaccharomyces pombe Rpa34/Sp-Rpa49 rescues growth defects in yeast mutants, confirming conserved roles .

Antibody Validation

  • Polyclonal antibodies (e.g., Boster Bio A30649) recognize recombinant Cd3eap in WB (1:500–1:2000 dilution), IHC (1:100–1:300), and ELISA (1:40,000) .

  • Epitope mapping confirms reactivity to residues 441–490 of human CD3EAP, with cross-reactivity in mouse and rat .

Disease Relevance

  • Cancer: Cd3eap overexpression correlates with elevated rRNA synthesis in proliferating cells, a hallmark of malignancies .

  • Immunology: Isoform 2 participates in preformed T-cell receptor (TCR) complexes, hinting at non-canonical roles in immune signaling .

Key Research Findings

  • Nucleolar Assembly: RPA34/RPA49 deletion in yeast collapses nucleolar structure, reversible only by reducing rRNA gene copy number .

  • Drug Sensitivity: Cd3eap-deficient cells exhibit hypersensitivity to mycophenolate and 6-azauracil, linking Pol I integrity to nucleotide metabolism .

  • Structural Analysis: Cryo-EM studies suggest RPA34 stabilizes Pol I–rDNA interactions during elongation .

Product Specs

Form
Lyophilized powder
Note: We prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order notes. We will fulfill your request if possible.
Lead Time
Delivery time may vary based on your purchasing method and location. Please consult your local distributors for specific delivery details.
Note: Our standard shipping includes normal blue ice packs. If you require dry ice shipment, please inform us in advance. Additional fees may apply.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging the vial briefly before opening to ensure the contents settle at the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We suggest adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%. Customers can use this as a reference.
Shelf Life
The shelf life is influenced by various factors including storage conditions, buffer composition, temperature, and the intrinsic stability of the protein.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process.
We will determine the tag type during production. If you have a specific tag type preference, please inform us, and we will prioritize developing it accordingly.
Synonyms
Cd3eap; Ase1; Paf49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Anti-sense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-399
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Cd3eap
Target Protein Sequence
MAGTQACSAT RFSCPPHFTE MSPDSEPSRF SLEALTGPDT ELWLIQAPAD FAPQCLNGRR VPLSGSKTVK GKLDGKKHRY RVFTSSPQAR EATLLASSSE AGGRLTCAPA PSGSLRIMEG PQEYLLSRVP LQLIPTSLPP QIPAGLRPRF SAFGGSPPVT GPGSASALRS PTSGKRKSTR KGTDASSDTQ EAVNRHGAME VKTALGNLGV SVKKRKRYFM QEEMEAKTME PVAELPVPSA TSSKKRKKSK GTETSQVEHT EPVAQTEPPE GTFLFPTKKR KRQKEADGTE EVDGIVADSQ PQVIVEAQEE TILLSPTKKR RKEKRQNLGM EAEMGPPGVL MVTEHSEHGL QAEVALVSPK KTKKKKGKRV GETEAALPDD FEPQGALAPS KKKERGARL
Uniprot No.
Q76KJ5

Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a component of RNA polymerase I, which synthesizes ribosomal RNA precursors. This enzyme is involved in UBTF-activated transcription, likely at a step following preinitiation complex formation.
Gene References Into Functions
  1. Yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric complexes interact through species-specific sequence elements that appear to have similar structural motifs. PMID: 22849406
  2. These findings strongly suggest that RNA polymerase I (Pol I)-associated factor PAF49/ASE-1 plays a crucial role in rRNA transcription. PMID: 15226435
  3. Serial deletion analysis revealed that amino acids 199-338 were essential for the nucleolar localization of PAF49. PMID: 18329376
Database Links

KEGG: mmu:70333

STRING: 10090.ENSMUSP00000044653

UniGene: Mm.171370

Protein Families
Eukaryotic RPA34 RNA polymerase subunit family
Subcellular Location
Nucleus, nucleolus. Note=Accumulates in the nucleolus of exponentially growing cells.

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