Recombinant Mouse Fibronectin type III and SPRY domain-containing protein 1 (Fsd1)

Shipped with Ice Packs

In Stock

Cat. No.

BT1158211

Source

Yeast

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT1158211

Source

E.coli

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT1158211

Source

E.coli

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT1158211

Source

Baculovirus

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT1158211

Source

Mammalian cell

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Purity

>85% (SDS-PAGE)

Quick Inquiry

Description

Production and Purification

Parameter	Details
Host Systems	Expressed in E. coli, yeast, baculovirus, or mammalian cell systems .
Purification Tags	Commonly uses His tag, GST tag, or Strep Tag for affinity chromatography .
Purity	≥85% as determined by SDS-PAGE .
Concentration	Quantified via absorbance at 280 nm using Expasy's ProtParam tool .

Applications in Research

Cell Adhesion Studies: Utilized to investigate integrin-mediated signaling due to its Fibronectin Type III domain .
Protein Interaction Mapping: The SPRY domain enables studies of binding partners in signaling cascades .
Structural Biology: Serves as a model for analyzing domain orientation effects on integrin affinity (e.g., RGD motif accessibility) .

Research Gaps and Opportunities

While structural data and production protocols are well-documented , functional studies specific to mouse Fsd1 remain limited. Current knowledge extrapolates from:

Human fibronectin fragments (e.g., roles in osteoblast mineralization and autophagy) .
SPRY domain functions in other proteins (e.g., immune regulation) .

Further studies could explore its role in tissue repair, cancer metastasis, or metabolic regulation, leveraging recombinant Fsd1’s modular domains .

Product Specs

Form

Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized preparation.

Lead Time

Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline for your preparations.

Shelf Life

Shelf life depends on several factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

Fsd1Fibronectin type III and SPRY domain-containing protein 1

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-496

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Mus musculus (Mouse)

Target Names

Fsd1

Target Protein Sequence

MEDQREALRK IITTLAMKNE ETQTFIYSLK QMLLNVEANS AKVQEDLEAE FQSLTSVLEE LKESMLMKIK QDRASRTYEL QNQLAACTRA LESSEELLET ANQTLQASDS EDFSQAAKEI KDGITMAPAF RLSLKAKVSD NMSHLMVDFA QERQMLQALK FLPVPSAPTI DLAESLVSDN CVTLVWHMPD EDSKIDHYVL EYRKTNFEGP PRLKEDHPWM VVEGIRQTEH TLTGLKFDMK YMNIRVKACN KAVAGEFSEP VTLETPAFMF RLDGSTSHQN LRVEDLSAEW DAMGGKVQDI KAREKEGKGR TASPVNSPAR GTPSPKRMSS GRGGRDRFTA ESYTVLGDTL IDGGEHYWEV RFEPDSKAFG LGVAYRSLGR FEQLGKTAAS WCLHANNWLQ ASFTAKHANK VKVLDSPVPD CLGVHCDFHQ GLLSFYNART KQLLHTFKAK FTQPLLPAFT VWCGSFQVTT GLQVPSAVRC LQKRGSATSS SNTSLT

Uniprot No.

Q7TPM6

Target Background

Function

May be involved in microtubule organization and stabilization.

Gene References Into Functions

Fibronectin extension and unfolding within cell matrix fibrils controlled by cytoskeletal tension. PMID: 11959962

Database Links

KEGG: mmu:240121

UniGene: Mm.270660

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cytoplasm. Cleavage furrow.

Q&A

Recombinant Mouse Fibronectin type III and SPRY domain-containing protein 1 (Fsd1) is a structurally complex protein involved in microtubule organization and cytokinesis, with applications in cellular biology and mechanistic studies. Below are FAQs organized by research complexity, incorporating methodological guidance and data-driven insights from peer-reviewed sources and technical documentation.

What are the critical structural domains of recombinant mouse Fsd1 and their functional implications?

Fsd1 contains three conserved domains:

B-box (BBC) domain: Mediates protein-protein interactions and subcellular localization .
Fibronectin type III domain: Facilitates extracellular matrix interactions and structural stabilization .
SPRY domain: Regulates signal transduction and microtubule binding .

Domain	Function	Experimental Validation Methods
B-box (BBC)	Centrosome association	Immunofluorescence, co-immunoprecipitation
Fibronectin	Structural stability	Circular dichroism, thermal shift assays
SPRY	Microtubule interaction	In vitro microtubule polymerization assays

Methodological Note: Domain-specific functional studies require tag-free or cleavable-tag protein variants to avoid steric interference .

Which expression systems are optimal for producing recombinant mouse Fsd1 with preserved activity?

Comparative data from multiple production systems:

Host System	Purity	Tag	Functional Validation Status
HEK-293 Cells	>90%	His/Strep	Partial activity in WB/ELISA
Wheat Germ	>80%	GST	Limited functional data
Cell-Free Synthesis	70-80%	Myc-DDK	Not validated

Recommendation: HEK-293 systems yield higher purity and are preferred for structural studies, while cell-free systems suit high-throughput screening .

How can researchers resolve discrepancies in Fsd1’s reported microtubule-stabilizing activity across studies?

Common sources of contradiction and solutions:

Issue	Resolution Strategy
Tag interference	Use cleavable tags (e.g., TEV protease sites)
Batch variability	Validate via analytical SEC/HPLC and SDS-PAGE
Assay specificity	Employ orthogonal methods (e.g., TIRF microscopy + co-sedimentation assays)

Case Example: A 2025 study using Strep-tagged Fsd1 reported reduced microtubule affinity compared to His-tagged variants, highlighting tag-dependent artifacts .

What strategies are effective for probing Fsd1’s role in cytokinesis using recombinant protein?

A phased experimental framework:

In vitro reconstitution: Combine Fsd1 with purified tubulin and monitor microtubule dynamics via live imaging .
Domain truncation: Generate ΔBBC or ΔSPRY mutants to identify critical regions for cytokinesis .
Crosslinking-MS: Map interaction partners in mitotic cell lysates .

Key Consideration: Functional studies require ultracentrifugation-purified Fsd1 (>95% purity) to exclude chaperone contaminants .

How should researchers validate Fsd1 stability under diverse experimental conditions?

Stability profiling parameters:

Condition	Stability Threshold	Analytical Method
Temperature (-80°C)	12 months	Size-exclusion chromatography
Freeze-thaw cycles	≤3 cycles	Western blot (anti-tag)
pH (6.0–8.0)	Retains >80% activity	Intrinsic fluorescence

Protocol Note: Buffer composition (e.g., 20 mM HEPES, 150 mM NaCl) is critical for preventing aggregation .

Table 1: Antibody Validation for Fsd1 Studies

Application	Recommended Controls	Citation
Western Blot	100x molar excess of Fsd1 fragment (aa 252-341)
IHC/ICC	siRNA knockdown + rescue with WT/mutant Fsd1

Table 2: Ortholog Cross-Reactivity

Species	Sequence Identity	Functional Conservation
Human	93%	Microtubule binding
Rat	93%	Cytokinesis
Zebrafish	82%	Developmental defects

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

Recombinant Mouse Fibronectin type III and SPRY domain-containing protein 1 (Fsd1)

Description

Production and Purification

Applications in Research

Research Gaps and Opportunities

Product Specs

Target Background

Q&A

What are the critical structural domains of recombinant mouse Fsd1 and their functional implications?

Which expression systems are optimal for producing recombinant mouse Fsd1 with preserved activity?

How can researchers resolve discrepancies in Fsd1’s reported microtubule-stabilizing activity across studies?

What strategies are effective for probing Fsd1’s role in cytokinesis using recombinant protein?

How should researchers validate Fsd1 stability under diverse experimental conditions?

Table 1: Antibody Validation for Fsd1 Studies

Table 2: Ortholog Cross-Reactivity

Quick Inquiry

Category

Service