Recombinant Mouse Formyl peptide receptor-related sequence 6 (Fpr-rs6)
Description
Immune Regulation
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Detects pathogen-associated molecular patterns (PAMPs) like formylated peptides, aiding bacterial clearance .
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Modulates corticotrophin (ACTH) secretion in the pituitary gland, linking innate immunity to neuroendocrine functions .
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Interacts with heme-binding protein 1 (Hebp1) to manage oxidative stress responses .
Chemosensory Functions
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Expressed in mouse vomeronasal organ (VNO) neurons at levels comparable to V1R/V2R chemoreceptors (3.7 neurons per 14 μm section) .
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Does not co-express with V1R/V2R receptors, suggesting independent chemosensory pathways .
Research Tools
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ELISA Kits: Quantify Fpr-rs6 in tissue homogenates (0.156–10 ng/ml detection range) .
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Interaction Studies: STRING database links Fpr-rs6 to Hebp1 (confidence score: 0.738) .
Key Research Findings
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Inflammation Resolution: Fpr-rs6 activation inhibits NF-κB signaling, reducing IL-1β, IL-6, and TNF-α in chorioamnionitis models .
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Glucocorticoid Modulation: Co-expressed with Fpr-rs1/rs2 in the anterior pituitary, mediating annexin A1-dependent anti-inflammatory effects .
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Pathogen Sensing: Detects formylated peptides from Listeria monocytogenes and mitochondria-derived motifs .
Technical Considerations
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Storage: Short-term at +4°C; long-term at -20°C to -80°C in PBS buffer .
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Customization: Available for expression systems, tag placement, and sequence optimization .
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Limitations: Requires 5–9 weeks for custom production; not validated for human diagnostics .
Unresolved Questions
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format during order placement for custom preparation.
Note: All proteins are shipped with standard blue ice packs unless dry ice is specifically requested in advance. Additional fees apply for dry ice shipping.
If you require a specific tag, please inform us, and we will prioritize its inclusion in the production process.
Target Background
Q&A
Technical Methodology
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What are the optimal conditions for expressing and purifying functional Fpr-rs6 from different host systems?
Optimal conditions for expressing and purifying functional Fpr-rs6 vary significantly across expression systems:
Expression System Optimal Conditions Purification Strategy Special Considerations Cell-Free Expression Temperature: 25-30°C
Reaction time: 4-6 hours
Reducing environmentAffinity chromatography with His- or GST-tag Requires optimization of redox conditions for proper folding E. coli Induction: 0.1-0.5 mM IPTG
Temperature: 16-20°C
Duration: 16-20 hoursInclusion body solubilization followed by refolding; affinity purification Often produces inclusion bodies; may require fusion partners Yeast Induction: 0.5-2% methanol (P. pastoris)
Temperature: 25-28°C
Duration: 48-72 hoursAffinity chromatography followed by size exclusion Monitor glycosylation patterns for consistency Baculovirus MOI: 1-5
Temperature: 27°C
Harvest: 48-72 hours post-infectionMembrane extraction with detergents followed by affinity purification Requires careful selection of detergents to maintain functionality Mammalian Cell Transient or stable expression
Temperature: 37°C
5% CO₂
Harvest: 48-72 hoursGentle membrane solubilization followed by affinity and ion exchange chromatography Most physiologically relevant but lowest yields Key purification considerations for maintaining Fpr-rs6 functionality include:
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Detergent selection for membrane protein extraction (e.g., DDM, LMNG, or CHS-containing mixtures)
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Buffer composition (typically pH 7.4 with physiological salt concentration)
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Addition of stabilizing agents during purification (glycerol, specific lipids)
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Temperature control throughout the purification process (4°C recommended)
Regardless of the expression system used, the target purity should be ≥85% as assessed by SDS-PAGE for most research applications .
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What experimental design considerations are essential when comparing Fpr-rs6 with other formyl peptide receptors?
When designing experiments to compare Fpr-rs6 with other formyl peptide receptors, researchers should address several critical considerations:
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Expression Standardization: Ensure comparable expression levels across different receptor subtypes through:
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Quantitative flow cytometry for cell surface expression
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Western blotting with epitope tags for total expression
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Binding site titration with saturating ligand concentrations
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Experimental Controls:
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Include positive control ligands known to activate specific receptor subtypes
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Use receptor-null cells as negative controls
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Include cross-desensitization experiments to distinguish receptor-specific effects
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Ligand Selection:
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Test a diverse panel of formylated and non-formylated peptides
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Include receptor-selective agonists and antagonists when available
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Use concentration-response curves rather than single concentrations
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Data Analysis Approaches:
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Apply appropriate statistical methods for comparing potency (EC₅₀/IC₅₀) and efficacy
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Use multivariate analysis to identify patterns in receptor response profiles
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Consider bias factor calculations for comparing signaling pathway activation
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Experimental Design Structure:
Example design matrix for systematic receptor comparison:
Receptor Ligand 1 Ligand 2 Ligand 3 ... Ligand n Fpr-rs6 EC₅₀, Emax EC₅₀, Emax EC₅₀, Emax ... EC₅₀, Emax Fpr1 EC₅₀, Emax EC₅₀, Emax EC₅₀, Emax ... EC₅₀, Emax Fpr2 EC₅₀, Emax EC₅₀, Emax EC₅₀, Emax ... EC₅₀, Emax Fpr-rs1 EC₅₀, Emax EC₅₀, Emax EC₅₀, Emax ... EC₅₀, Emax This systematic approach enables meaningful comparison of pharmacological profiles across receptor subtypes while minimizing experimental biases .
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