Recombinant Mouse G2/M phase-specific E3 ubiquitin-protein ligase (G2e3)

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Cat. No.
BT2404290
Source
Yeast
Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2404290
Source
E.coli
Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2404290
Source
E.coli
Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2404290
Source
Baculovirus
Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2404290
Source
Mammalian cell
Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Purity
>85% (SDS-PAGE)
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Description

Introduction to Recombinant Mouse G2/M Phase-Specific E3 Ubiquitin-Protein Ligase (G2e3)

Recombinant Mouse G2/M phase-specific E3 ubiquitin-protein ligase, commonly referred to as G2e3, is a crucial enzyme involved in the ubiquitination process. This process is vital for protein degradation, cell cycle regulation, and DNA damage response. G2e3 is particularly significant in early embryonic development, where it prevents apoptosis and ensures proper cell division.

Role in Early Embryonic Development

G2e3 plays a critical role in early embryonic development. Knockout mouse models have shown that embryos lacking G2e3 die before implantation due to massive apoptosis, despite normal blastocyst formation at embryonic day 3.5 . This highlights G2e3's essential function in preventing apoptosis during early embryogenesis.

Expression and Localization

G2e3 is predominantly expressed in the central nervous system and early limb bud formation during embryonic development. In adult mice, it is intensely expressed in Purkinje cell bodies and cells lining the ductus deferens . This specific localization suggests that G2e3 may have additional roles beyond embryonic development.

Research Findings and Implications

Recent studies have explored the role of ubiquitin ligases, including G2e3, in various biological processes. For instance, G2e3 has been identified as a potential target for chemosensitizing cancer cells due to its involvement in DNA damage response pathways . Additionally, research on the Chinese Tongue Sole has highlighted the importance of similar E3 ubiquitin ligases in gametogenesis, suggesting potential applications in aquaculture .

Data Tables

While specific data tables for recombinant mouse G2e3 are not readily available, research findings can be summarized as follows:

AspectDescription
StructureDual RING-like domains; catalytically inactive HECT domain .
FunctionEssential for preventing apoptosis in early embryonic development .
ExpressionPredominantly in central nervous system and limb buds during embryogenesis; intense in Purkinje cells and ductus deferens in adults .
InteractionsSpecifically interacts with UbcH5a for ubiquitination .

References https://pmc.ncbi.nlm.nih.gov/articles/PMC2494922/ https://pmc.ncbi.nlm.nih.gov/articles/PMC11394658/ https://pmc.ncbi.nlm.nih.gov/articles/PMC4359243/ https://www.ncbi.nlm.nih.gov/gene/217558 https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=55632 https://pmc.ncbi.nlm.nih.gov/articles/PMC2736652/ https://www.antibodies-online.com/protein/3080424/G2+M-Phase+Specific+E3+Ubiquitin+Protein+Ligase+G2E3+AA+1-706+protein+Strep+Tag/ https://rgd.mcw.edu/rgdweb/report/gene/main.html?id=1310263

Product Specs

Form
Lyophilized powder

Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this during order placement.

Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for specific delivery timelines.

Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.

Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.

The tag type will be determined during production. If you require a specific tag type, please inform us, and we will prioritize its development.

Synonyms
G2e3; Kiaa1333G2/M phase-specific E3 ubiquitin-protein ligase; EC 2.3.2.26; G2/M phase-specific HECT-type E3 ubiquitin transferase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-716
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
G2e3
Target Protein Sequence
MNENKPDNSQ SLACVFCRKN DDCPNKYGEK KTYEKWNFSV HYYCLLMSSG IWQRGKEEEG VYGFLIEDIR KEVQRASKLK CTVCKKNGAS IGCVVPTCKR SYHLPCGLQK ECIFQFTDNF ASFCWKHRPV QAITSNKYSS SLPCTICLEF VEPIPTYNIL QSPCCKNAWF HRDCLQVQAI NAGVFFFRCT LCNNTDIFQK EMLRMGIHIP EKDASWELEE NAYQELLQSH DRCDIRRCHC KKGRDYNEPN SKWEVKRCQS CGSSGTHLAC SSLQSWEQNW ECLDCRRITY TSDFQKAPKH PLANSTNVTV TDCLLEESSS KLPRQSTVAQ HKELLRQGSK FRRDISTILI ELGFQIKKKT KTLYINKANV WRSALEQFQS QKFNPSCSID VVYVNGNEVG SQHLGSKQEF LSHLMHHLEN SSVFEGSLAK NLSLNSQAVK ENLYYEVGKM LAISLVHGGP SPGFFSETLF NCLAYGPENT LPTLDDVSDI DVAQIIIKID SATDLNILNS VISQHYNYLE VSGCLRLTTS LSDKFMLVKD ILFYHVINRV KAPFESFKQG LKTLGVLEKI QTYPEAFYKI LCHKPENLSA KNLSDLFTIH SVADVQTLRF WNSYLKAIED GKSATTMEDI LIFATGCSSV PPTGFKPSLS VECLHVDFPV ADKYRNHLVL PATNTYEEFQ ENMDFTIRDT LRLEKEERSH ILPRTLNVSS NEEMLI
Uniprot No.
Q5RJY2

Target Background

Function

This protein is an E3 ubiquitin-protein ligase. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme as a thioester and directly transfers the ubiquitin to target substrates. It plays a crucial role in preventing apoptotic cell death during early embryogenesis.

Gene References Into Functions
  1. G2E3 is a dual-function ubiquitin ligase essential for preventing apoptosis in early embryogenesis. PMID: 18511420
Database Links

KEGG: mmu:217558

STRING: 10090.ENSMUSP00000113270

UniGene: Mm.253264

Subcellular Location
Nucleus, nucleolus. Cytoplasm.
Tissue Specificity
In the developing embryo, expressed predominantly in the central nervous system and early limb bud. In the adult, highest expression in Purkinje cell bodies and cells lining the ductus deferens.

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