Recombinant Mouse Immunoglobulin superfamily DCC subclass member 3 (Igdcc3)

Introduction to Igdcc3

Immunoglobulin Superfamily DCC Subclass Member 3 (Igdcc3) is a member of the immunoglobulin superfamily proteins and specifically belongs to the DCC (Deleted in Colorectal Cancer) subclass. In mice, the Igdcc3 gene is officially designated as "immunoglobulin superfamily, DCC subclass, member 3" . The protein is encoded by a gene located on mouse chromosome 9 and is characterized by its immunoglobulin-like domains, which are common structural features in many cell surface proteins involved in cell adhesion, receptor signaling, and molecular recognition processes.

Igdcc3 has been identified as a protein of interest in several biological contexts, particularly in cerebrovascular research where alterations in its expression levels have been associated with pathological vascular remodeling. As a member of the immunoglobulin superfamily, it likely participates in cell-cell interactions and signaling pathways that influence tissue structure and function.

Role in Vascular Biology

Recent research has implicated Igdcc3 in vascular biology, particularly in processes related to vascular remodeling. In a proteome profiling study of brain vessels in a mouse model overexpressing transforming growth factor beta (TGFβ) in the brain, Igdcc3 was identified among proteins showing significantly altered levels compared to wild-type mice . Specifically, Igdcc3 showed increased expression in the cerebral arteries of these TGF mice, which are characterized by cerebrovascular pathology similar to that seen in vascular cognitive impairment and dementia (VCID) .

The study identified Igdcc3 as one of 11 proteins (out of 20 with significantly altered levels) known to influence vascular remodeling. Other proteins in this group included Dll3, Adgrg2, Krt24, Ptprd, Ptprn2, Rngtt, Rp1l1, Tacstd2, Zfyve26, and Zfyve27 . This association suggests that Igdcc3 may play a role in the structural modifications of blood vessels that occur during both normal development and pathological conditions.

Association with Cerebrovascular Pathology

The TGF mouse model used in the aforementioned study recapitulates several features of cerebrovascular pathology observed in VCID, including thickened vascular walls, impaired dilatory function, cerebral hypoperfusion, neurovascular uncoupling, and cerebral microhemorrhages . The observation that Igdcc3 levels are altered in this model suggests a potential involvement in the pathological processes underlying these vascular abnormalities.

Furthermore, the study confirmed the presence of Igdcc3 in depleted human plasma samples, indicating that this protein may be detectable in the circulation and potentially serve as a biomarker for cerebrovascular conditions . This finding opens up possibilities for non-invasive diagnostic approaches based on the detection of Igdcc3 or its fragments in blood samples.

Recombinant Forms and Production

For research applications, Recombinant Mouse IGDCC3 Protein is commercially available with various tags to facilitate purification and detection. One such product is Recombinant Mouse IGDCC3 Protein with His (Fc)-Avi tag, which is expressed and purified from HEK293 cells . HEK293 cells are a widely used mammalian expression system that generally provides proper folding and post-translational modifications for mammalian proteins.

Several commercial variants of recombinant mouse Igdcc3 are available, including:

• Recombinant Mouse IGDCC3 Protein (untagged)

• Recombinant Mouse IGDCC3 Protein, His (Fc)-Avi-tagged

• Recombinant Full Length Mouse Immunoglobulin Superfamily Dcc Subclass Member 3 (Igdcc3) Protein, His-Tagged

Additionally, human variants such as Recombinant Human IGDCC3, His-tagged are also commercially available, facilitating comparative studies between mouse and human forms of the protein .