Recombinant Mouse JNK1/MAPK8-associated membrane protein (Jkamp)
Description
Introduction to Recombinant Mouse JNK1/MAPK8-Associated Membrane Protein (JKAMP)
Recombinant Mouse JNK1/MAPK8-Associated Membrane Protein (JKAMP), also known as JAMP or C14orf100, is a critical membrane protein involved in regulating JNK1 (MAPK8) signaling. It plays pivotal roles in cellular processes such as stress response, apoptosis, and protein quality control, particularly in endoplasmic reticulum (ER)-associated degradation (ERAD) pathways . The recombinant form is engineered for research use, enabling precise study of its biochemical interactions and therapeutic potential.
Applications in Research and Diagnostics
3.1 Detection via ELISA
Mouse-specific ELISA kits (e.g., MODL00755) enable quantification of JKAMP in serum, plasma, and cell lysates. Key specifications include:
| Parameter | Mouse Kit (MODL00755) | Rat Kit (RTDL00598) |
|---|---|---|
| Sensitivity | 0.26 ng/mL | 0.61 ng/mL |
| Detection Range | 0.781–50 ng/mL | 1.56–100 ng/mL |
| Matrices | Tissue homogenates, cell culture supernatants |
These kits use sandwich ELISA formats with high intra-assay precision (CV <10%) .
3.2 Antibody-Based Studies
Antibodies targeting JKAMP or JNK1 are used for immunoblotting and immunohistochemistry. For example:
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Anti-JNK1 antibodies (e.g., clone GH05/4G10) detect JNK1 at ~44/52 kDa in Western blots .
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Recombinant JKAMP serves as a positive control in assays to validate antibody specificity .
Research Findings and Disease Implications
4.1 Role in Inflammatory Pathways
JKAMP/JNK1 signaling contributes to psoriasis pathogenesis by regulating cytokine production in myeloid cells and keratinocyte proliferation. Studies using Mapk8 knockout mice revealed reduced IL-23 and IL-1β levels, suggesting JKAMP’s role in inflammasome activation .
4.2 ER Stress and Protein Degradation
JKAMP facilitates ERAD by linking misfolded proteins to the proteasome. This function is critical in neurodegenerative diseases, where protein misfolding is a hallmark .
Production and Quality Control
5.1 Recombinant Expression
Mouse JKAMP is produced via bacterial systems with:
| Parameter | Details |
|---|---|
| Host | E. coli (prokaryotic) |
| Purity | >90% (SDS-PAGE confirmed) |
| Storage | -80°C (lyophilized) or 4°C (short-term) |
5.2 Cross-Reactivity Considerations
ELISA kits may show limited cross-reactivity with analogs, necessitating validation with native proteins .
Challenges and Future Directions
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Species-Specific Interactions: Rat and human JKAMP orthologs differ in sequence and function, requiring validation for cross-species studies .
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Therapeutic Targets: JKAMP’s role in ERAD and apoptosis makes it a candidate for cancer or neurodegenerative therapies, though further in vivo studies are needed .
Product Specs
Note: We will prioritize shipping the format that we have in stock. However, if you have any specific requirements for the format, please indicate your needs in the order remarks, and we will fulfill them accordingly.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
- JAMP serves as a membrane-anchored regulator of JNK1 activity duration in response to various stress stimuli [JAMP, a Jun N-terminal kinase 1 (JNK1)-associated membrane protein] PMID: 16166642
- JAMP is crucial for the coordinated clearance of misfolded proteins from the endoplasmic reticulum. PMID: 18784250
