Recombinant Mouse Kelch repeat and BTB domain-containing protein 11 (Kbtbd11)

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Cat. No.
BT2434736
Source
Yeast
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434736
Source
E.coli
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434736
Source
E.coli
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434736
Source
Baculovirus
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434736
Source
Mammalian cell
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Purity
>85% (SDS-PAGE)
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Description

Introduction to Recombinant Mouse Kelch Repeat and BTB Domain-Containing Protein 11 (Kbtbd11)

Recombinant Mouse Kelch repeat and BTB domain-containing protein 11 (Kbtbd11) is a protein belonging to the BTB-Kelch family, characterized by its BTB/POZ domain and Kelch repeats. This protein is involved in various cellular processes, including protein-protein interactions, ubiquitination, and cell differentiation. The BTB domain facilitates dimerization and interactions with other proteins, while the Kelch repeats form a β-propeller structure that acts as a scaffold for protein interactions .

Functional Roles:

  • Adipogenesis: Kbtbd11 plays a significant role in adipocyte differentiation. Its expression increases during adipogenesis, and it is regulated by transcription factors like PPARγ and USF1. Knockdown of Kbtbd11 suppresses adipocyte differentiation, while overexpression promotes it .

  • Osteoclastogenesis: Kbtbd11 acts as a negative regulator of osteoclast differentiation by controlling the ubiquitination and degradation of NFATc1 through interaction with Cullin3 .

Adipocyte Differentiation:

ConditionEffect on Adipocyte Differentiation
Kbtbd11 KnockdownSuppresses differentiation
Kbtbd11 OverexpressionPromotes differentiation

Kbtbd11 is crucial for the early stages of adipocyte differentiation, particularly during mitotic clonal expansion (MCE). Its expression is enhanced in mice fed a high-fat diet compared to those on a standard chow diet .

Osteoclast Differentiation:

ConditionEffect on Osteoclast Differentiation
Kbtbd11 KnockdownEnhances osteoclast formation
Kbtbd11 OverexpressionImpairs osteoclast differentiation

Kbtbd11 negatively regulates osteoclast differentiation by promoting the ubiquitination and proteasomal degradation of NFATc1, a key factor in osteoclastogenesis .

Protein Interactions

Kbtbd11 interacts with several proteins, including HSC70 and HSP60, which may influence its stability or folding. Inhibitors of these proteins increase Kbtbd11 protein levels, suggesting a role in its regulation .

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
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Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.
The tag type is determined during production. If a specific tag type is required, please inform us for preferential development.
Synonyms
Kbtbd11; Kiaa0711; Kelch repeat and BTB domain-containing protein 11
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-633
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Kbtbd11
Target Protein Sequence
MENSVAPFVL YSGTEPRTPG EDSLPLPAEE EGAASTAQTP CSLSASLCFS SGDDSPPQSR ASAAEGSEAS PPSLRSDLRV VETQWDVSSA ASPESPEECA RPEEPASPED PPSRHEHARP VELESLDELG EPVPVPPGVG SVHGEPDLVI EVAGRRLRAH KAVLAARSDY FRARASRDVL RVQGVSFTAL RLLLADAYSG RMAGVRPDNV AEVVAGARRL QLPGAAQRAT EAMAPQLSLD NCYEVLSAGK RQRLTELRDA AYRFMSDHYL EVLREPAVFG RLSGAERDLL LRRRLCTGRA CLLAAALGTT GERSGSRPQS PSGDAESRGD AAVYCYQAEA GEWRELTRLP EGAPARGCGL CVLFNYLFLA GGVAPAGPDG RARPSDQVYC YNPVTDSWST VRPLRQARSQ VQLLALDGHL YAVGGECLLS VERYDPRADR WTAVAPLPRG AFAVAHEAAT CNGEIYVSGG SLFYRLLKYD PRRDEWQECP CSSSRERSAD MVALDGFLYR FDLCGSRGEA QAAVGSGGGV SVFRYHCLAK QWSQCAVHLR PPGAPAGLQP FRCVALDGTI YCVSRAGTWR FVPSQDTEAG SDMGPGGSFE PEPLGSPLDV RGVLFPFVLN LPEKPDRGEQ GAV
Uniprot No.
Q8BNW9

Q&A

FAQs for Researchers: Recombinant Mouse Kbtbd11

Advanced Research Questions

  • How does Kbtbd11 mechanistically regulate NFATc1 in osteoclastogenesis?
    Kbtbd11 recruits Cullin3 to form an E3 ubiquitin ligase complex, promoting NFATc1 polyubiquitination at Lysine 684. This targets NFATc1 for proteasomal degradation, reducing its half-life from 8h to <2h . Key validation steps:

    • Co-immunoprecipitation (Co-IP) with anti-Cullin3 antibodies

    • Cycloheximide chase assays + MG132 treatment to measure degradation kinetics .

  • Why does Kbtbd11 overexpression fail to alter lipid accumulation in mature adipocytes?
    Despite 80-fold mRNA upregulation, Kbtbd11 does not affect Pparγ or aP2 in differentiated 3T3-L1 cells . This suggests:

    • Role restricted to mitotic clonal expansion (MCE) phase, not terminal differentiation

    • Compensatory mechanisms by parallel pathways (e.g., USF1 or MYC) .
      Experimental design tip: Time overexpression to days 0–2 of adipogenesis for functional studies .

  • How to resolve contradictory data on Kbtbd11’s role in cancer vs. metabolic tissues?
    Tissue-specific interactions explain discrepancies:

    ContextFunctionKey Partners
    Colorectal cancerTumor suppressor (↓ in tumors) MYC
    AdipocytesPro-differentiation USF1, PPARγ
    OsteoclastsAnti-differentiation Cullin3, NFATc1
    Use context-specific KO models and ChIP-seq to map binding targets .

Methodological Challenges

  • Why does Kbtbd11 antibody produce nonspecific bands in Western blot?
    The BTB domain shares homology with KBTBD family members (e.g., KBTBD2/6). Mitigate via:

    • Pre-adsorption with recombinant Kbtbd11

    • Combine siRNA knockdown with blotting to confirm band specificity .

  • How to optimize Kbtbd11 promoter-reporter assays?
    The 2.3-kb mouse promoter and 1.7-kb human promoter show maximal USF1 responsiveness . Critical elements:

    • Ebox motif at −796 bp (mouse) and −502 bp (human)

    • Co-transfect with USF1 expression vector for 5–7x activation .

Key Citations

  • NFATc1 degradation mechanism

  • USF1 transcriptional regulation

  • Diet-dependent expression profiles

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