Recombinant Mouse N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein (Piga), partial
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipments.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipments.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
Note: If a specific tag type is required, please inform us, and we will prioritize its development.
Note: If a specific tag type is required, please inform us, and we will prioritize its development.
Synonyms
Piga; N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein; PIG-A
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Piga
Uniprot No.
Target Background
Function
The catalytic subunit of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex. It catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, initiating GPI biosynthesis.
Gene References Into Functions
- The reliability of using GPI anchor-deficiency as a valid reporter of Pig-a gene mutation. PMID: 29115020
- The assay's utility for environmental agent mutagenicity screening and *in vitro* hypothesis testing prior to *in vivo* Pig-a assays. PMID: 29098723
- Pig-a mutations identified in all clones from CD90-deficient cells, absent in CD90-proficient cells. B[a]P-induced Pig-a mutations predominantly involved base pair substitutions, small insertions, and deletions at G:C rich sequences. PMID: 29150045
- Diet-induced obesity increases Pig-a mutant erythrocyte frequency in male C57BL/6J mice. PMID: 27739633
- Reduced IgE/antigen-mediated passive cutaneous anaphylaxis in mice with Piga-deficient mast cells. PMID: 21865342
- Hematopoietic cell Pig-a gene deletion doesn't cause marrow failure but leads to clonally-restricted, inactive yet functionally competent CD8 T cells. PMID: 19679885
- Piga gene mutation doesn't alter cell death susceptibility in thymocytes, granulocytes, and hematopoietic progenitor cells. PMID: 12488505
- Pig-a knockout females exhibit infertility due to unfertilized eggs post-mating. PMID: 12692150
- Lack of this protein doesn't cause bone marrow progenitor cell retention. PMID: 15297852
- Impaired profilaggrin processing to its monomeric form in Pig-a null mouse epidermis. PMID: 15304084
- Piga expression is crucial for proper hair cell orientation in the inner ear, a process regulated by the planar cell polarity pathway. PMID: 19762422
Database Links
Protein Families
Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein.
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