Recombinant Mouse NEDD4 family-interacting protein 2 (Ndfip2)

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Cat. No.

BT2483011

Source

in vitro E.coli expression system

Synonyms

Ndfip2; Kiaa1165; N4wbp5A; NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; Fragment

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Product Specs

Form

Lyophilized powder
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Lead Time

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Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.

Shelf Life

Shelf life depends on several factors: storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.
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Synonyms

Ndfip2; Kiaa1165; N4wbp5A; NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; Fragment

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-311

Protein Length

full length protein

Species

Mus musculus (Mouse)

Target Names

Ndfip2

Target Protein Sequence

RRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTP ARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDP DSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAP QRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAI CGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMA AAHRTRYFFLL

Uniprot No.

Q91ZP6

Target Background

Function

NEDD4 family-interacting protein 2 (NDFIP2) activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1, and WWP2. This activation modulates the stability of their target proteins, influencing various cellular processes. NDFIP2 recruits ITCH, NEDD4, and SMURF2 to endosomal membranes. It negatively regulates KCNH2 potassium channel activity by reducing cell-surface expression and interfering with channel maturation. This interference involves recruiting NEDD4L to the Golgi apparatus and multivesicular bodies, where KCNH2 degradation is mediated. NDFIP2 may also modulate EGFR signaling. In conjunction with NDFIP1, it limits cytokine signaling and effector Th2 T-cell expansion by promoting JAK1 degradation, likely through ITCH- and NEDD4L-mediated ubiquitination.

Database Links

STRING: 10090.ENSMUSP00000137875

UniGene: Mm.290669

Subcellular Location

Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Endosome, multivesicular body membrane; Multi-pass membrane protein.

Tissue Specificity

Ubiquitously expressed, with highest levels in brain, liver, kidney and testis.

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Recombinant Mouse NEDD4 family-interacting protein 2 (Ndfip2)

Product Specs

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