Recombinant Mouse Phosphatidylinositol N-acetylglucosaminyltransferase subunit Q (Pigq)
Description
Function in GPI Biosynthesis
Pigq is part of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex, which catalyzes the first step in GPI biosynthesis: transferring N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI) . This reaction forms GlcNAc-PI, a precursor for GPI anchors.
Role in the Enzyme Complex
The GPI-GnT complex includes six core subunits in mammals:
Pigq interacts directly with PIGA and PIGC, stabilizing the complex and enabling enzymatic activity . In S. cerevisiae, a homologous subunit (Eri1p) regulates GPI-GnT activity, but no direct functional linkage to Ras GTPases has been confirmed in mammals .
Production and Purification
Disease Associations
Mutations in Pigq are linked to Multiple Congenital Anomalies-Hypotonia-Seizures Syndrome 4 (MCAHS4) and epilepsy, underscoring its role in neuronal development and function .
Experimental Uses
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Enzyme Activity Assays: Studies on GPI-GnT kinetics and subunit interactions .
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Structural Biology: Crystallization and X-ray diffraction studies to map catalytic sites .
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Disease Modeling: Knockout models to explore GPI deficiency phenotypes .
Interactions and Complex Formation
Pigq forms stable interactions with:
These interactions ensure proper localization of the complex to the ER membrane and coordination of enzymatic activity .
Clinical and Research Significance
Pigq serves as a critical tool for:
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them during order placement. We will accommodate your needs to the best of our ability.
Note: Our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate this in advance, as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
