Recombinant Mouse Probable low affinity copper uptake protein 2 (Slc31a2)
Description
Biological Function and Mechanism
Slc31a2 belongs to the solute carrier family 31 (SLC31) of copper transporters. Key functional insights include:
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Copper Homeostasis: Facilitates low-affinity copper uptake and mobilizes intracellular copper stores from endosomes/lysosomes .
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Regulatory Role: Modulates SLC31A1 (CTR1) activity by stabilizing its truncated form, influencing copper and cisplatin accumulation .
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Localization: Primarily resides in intracellular membranes (e.g., late endosomes, lysosomes) rather than the plasma membrane .
Studies in mice reveal that Slc31a2 expression decreases under copper supplementation, suggesting feedback regulation .
Production and Validation
Recombinant Slc31a2 is produced using E. coli expression systems, ensuring high yield and purity . Validation methods include:
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Western Blot: Detected in mouse pancreas using anti-Slc31a2 polyclonal antibodies (e.g., CAB16362) .
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ELISA: Quantifiable in tissue homogenates with a sensitivity range of 0.156–10 ng/ml .
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Functional Assays: Demonstrated copper transport activity in vitro, albeit with lower affinity compared to CTR1 .
Disease Modeling
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Copper-Related Disorders: Used to study Menkes disease and Wilson’s disease, where copper metabolism is disrupted .
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Cancer Research: Linked to cisplatin resistance; knockdown enhances cisplatin uptake in cancer cells .
Mechanistic Studies
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Subcellular Trafficking: Localization studies using immunofluorescence reveal its role in vesicular copper release .
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Gene Expression Analysis: RT-qPCR profiles show tissue-specific expression, with high levels in the liver and kidney .
Drug Development
Comparative Insights
Challenges and Future Directions
Product Specs
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Note: All proteins are shipped with standard blue ice packs unless dry ice is requested in advance. Additional fees apply for dry ice shipping.
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Target Background
- Increased granular staining and heparin content suggest an impact of Ctr2 on mast cell maturation. Supporting this, Ctr2 absence resulted in significantly increased tryptase mRNA expression, storage, and enzymatic activity. PMID: 26342034
- In mouse embryo fibroblasts, reduced CTR1 expression confers cisplatin resistance, while reduced CTR2 expression increases hypersensitivity both in vitro and in vivo. PMID: 24522273
- Ctr2 regulates the biogenesis of a cleaved mammalian Ctr1 metal transporter form lacking the copper- and cisplatin-binding ectodomain. PMID: 24167251
- Copper transporter 2 modulates cisplatin transport by controlling macropinocytosis rates. PMID: 20930109
