Recombinant Mouse Protein Dr1 (Dr1)

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Cat. No.
BT1250313
Source
Yeast
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1250313
Source
E.coli
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1250313
Source
E.coli
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1250313
Source
Baculovirus
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT1250313
Source
Mammalian cell
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Purity
>85% (SDS-PAGE)
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Product Specs

Form
Lyophilized powder
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please specify them during order placement, and we will accommodate your request.
Lead Time
Delivery time may vary depending on the purchasing method or location. For specific delivery time estimates, please consult your local distributor.
Note: Our proteins are standardly shipped with blue ice packs. If dry ice shipping is required, please inform us in advance as additional fees will apply.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend briefly centrifuging this vial before opening to ensure the contents settle to the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our default glycerol concentration is 50%. Customers can use this as a reference.
Shelf Life
Shelf life is influenced by various factors including storage conditions, buffer ingredients, temperature, and protein stability.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is necessary for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
Tag type is determined during production. If you have a specific tag type requirement, please inform us, and we will prioritize development of the specified tag.
Synonyms
Dr1; Protein Dr1; Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-176
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Dr1
Target Protein Sequence
ASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA STQAGSSQDE EDDDDI
Uniprot No.
Q91WV0

Target Background

Function
The DR1/DRAP1 heterodimer association with TBP results in functional repression of both activated and basal transcription of class II genes. This interaction prevents the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. DR1 can bind to DNA independently. It is a component of the ATAC complex, which possesses histone acetyltransferase activity on histones H3 and H4.
Gene References Into Functions
  1. Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 co-localize on chromatin and collaboratively suppress pervasive transcription in murine embryonic stem cells. PMID: 28735899
Database Links

KEGG: mmu:13486

STRING: 10090.ENSMUSP00000031190

UniGene: Mm.303534

Protein Families
NC2 beta/DR1 family
Subcellular Location
Nucleus.

Q&A

FAQs for Researchers on Recombinant Mouse Protein DDR1 (Dr1)
This collection addresses key methodological and analytical challenges in working with recombinant mouse DDR1, focusing on experimental design, data interpretation, and advanced applications. Questions are categorized into basic and advanced tiers, with evidence-based answers derived from peer-reviewed studies and technical specifications .

Advanced Research Questions

How to resolve discrepancies in collagen-binding kinetics between DDR1 isoforms?

  • Experimental design:

    • Isoform comparison: Compare full-length DDR1 (470 aa cytoplasmic domain) vs. splice variants lacking the NPxY motif .

    • Kinetic assays: Use SPR to measure on/off rates (e.g., DDR1–collagen I: kon ≈ 1.5 × 10^4 M⁻¹s⁻¹, koff ≈ 2 × 10⁻³ s⁻¹) .

    • Data contradiction analysis:

      • Issue: Variant isoforms show reduced binding due to disrupted oligomerization.

      • Solution: Include cross-linking agents (e.g., BS³) to stabilize dimers .

How to validate DDR1’s role in epithelial junction regulation?

  • Methodology:

    • Co-culture assays: Treat MDCK-II epithelial cells with 10 nM recombinant DDR1 and monitor E-cadherin redistribution via immunofluorescence .

    • Functional readouts:

      • Inhibition of collagen-induced DDR1 phosphorylation: Use phospho-tyrosine antibodies (e.g., pY513) .

      • MMP-2 activation: Measure gelatinase activity in conditioned media (≥2-fold increase in DDR1-overexpressing glioblastoma models) .

PhenotypeAssayExpected Outcome
Reduced cell migrationTranswell assay≤40% migration in DDR1-treated groups
Altered collagen fibrillogenesisSEM imagingDisorganized fibrils in DDR1 KO models

How to address low signal in DDR1 tetramer staining for T-cell studies?

  • Advanced solution:

    • Covalent peptide-MHC linkage: Engineer DR1 multimers with A12 analog peptides using (Gly)₄-Ser linkers to enhance stability .

    • Staining protocol:

      • Pre-incubate T cells with 50 μg/mL tetramer (4°C, 2 hr).

      • Use phycoerythrin-streptavidin amplification (e.g., 11,400 ± 392 MFI in collagen-specific hybridomas) .

What controls are essential for DDR1-dependent cytokine profiling?

  • Critical controls:

    • Wild-type vs. β2m⁻/⁻/IAβ⁻/⁻ mice: Confirm HLA-DR1-restricted responses (e.g., IFN-γ levels: 4–5-fold higher in HLA-A11/DR1 Tg mice) .

    • Cytokine multiplex panels: Include IL-4/IL-10 to identify suppressive T-cell subsets (e.g., A12-specific T cells show IL-4⁺/IL-10⁺/FoxP3⁻ phenotype) .

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