Recombinant Mouse Putative methyltransferase NSUN3 (Nsun3)

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Cat. No.
BT2454418
Source
Yeast
Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2454418
Source
E.coli
Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2454418
Source
E.coli
Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2454418
Source
Baculovirus
Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2454418
Source
Mammalian cell
Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Purity
>85% (SDS-PAGE)
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Product Specs

Form
Lyophilized powder

Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this in your order notes; we will fulfill your request to the best of our ability.

Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.

Note: All proteins are shipped with standard blue ice packs. Dry ice shipping is available upon request and incurs additional charges. Please contact us in advance to arrange this.

Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a reference.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer components, temperature, and the protein's inherent stability.

Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is recommended for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.

The specific tag type is determined during production. If you require a particular tag, please inform us, and we will prioritize its inclusion.

Synonyms
Nsun3; tRNA; cytosine(34)-C(5))-methyltransferase; mitochondrial; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 3
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-348
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Nsun3
Target Protein Sequence
MLTRLKAKSE GKLAKQLCRV VLDQFDKQYS KELGDSWSTV RDVLISPSLW QYAILFNRFN YPFELEKALH LRGYHTVLQG ALPHYPKSMK CYLSRTPDRM PSERHQTGSL KKYYLLNAAS LLPVLALELR DGEAVLDLCA APGGKSVALL QCAYPGYLLC NEYDRPRGRW LRQTLESFIP QPLINVIKVS ELDGREMGDA QPATFDKVLV DAPCSNDRSW LFSSDSQKAA YRIHQRKNLP VLQVELVRSA IKALRPGGLL VYSTCTLSKA ENQDVISEVL TSDSNIVPVD ISGIARTFSQ DFTFAPTDQK CSLLVIPEKG KAWGPMYIAK LKKGMSTRKR QGEFCKPC
Uniprot No.
Q8CCT7

Target Background

Function

NSUN3 is a mitochondrial tRNA methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). This methylation at the wobble position of the anticodon initiates the formation of 5-formylcytosine (f5C). The f5C modification at the wobble position expands codon recognition in mitochondrial translation, enabling mt-tRNA(Met) to recognize the AUA codon in addition to AUG.

Gene References Into Functions
  1. Nsun3 regulates embryonic stem cell differentiation by promoting mitochondrial activity. PMID: 29103146
Database Links

KEGG: mmu:106338

STRING: 10090.ENSMUSP00000059720

UniGene: Mm.26321

Protein Families
Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
Subcellular Location
Mitochondrion matrix.

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