Recombinant Mouse Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform (Ppp2r5a)
Description
Production and Purification
Recombinant mouse Ppp2r5a is typically produced in heterologous systems with the following specifications :
Cardiac Arrhythmia Mechanisms
Studies using PPP2R5A knockout (KO) mice reveal:
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Prolonged QT intervals and increased ventricular tachyarrhythmia under catecholaminergic stress
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Dysregulated Ca²⁺ handling: KO cardiomyocytes exhibit spontaneous Ca²⁺ transients, promoting arrhythmogenic activity
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Pathway linkage: PP2A-B56α deficiency reduces association with RyR2, increasing phosphorylation at serine-2814 and SR Ca²⁺ leak
Tumor Suppression
Recombinant B56α is pivotal in cancer studies:
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Hepatocellular carcinoma (HCC): Overexpression inhibits HCC cell proliferation (↓c-Myc, ↓Bcl-2) and metastasis via MAPK/AKT/WNT pathway suppression
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HBV interaction: HBV infection downregulates PPP2R5A, facilitating HCC progression by evading phosphatase-mediated tumor suppression
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Clinical correlation: Low PPP2R5A expression in Chinese HCC patients correlates with poor prognosis (p < 0.001)
Viral Pathogenesis
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Lentiviral infections: Accelerate PPP2R5A degradation to enhance viral replication
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HBV: Modulates PP2A-B56α localization to disrupt host cell cycle checkpoints
Research Applications
Limitations and Considerations
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Post-translational modifications: Recombinant proteins may lack native phosphorylation states
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Species specificity: Mouse PP2R5A antibodies show cross-reactivity with human samples but require validation
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Functional redundancy: B56β/γ subunits may compensate in knockout models, necessitating multi-isoform analyses
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you have any specific format requirements, please indicate them in your order notes, and we will fulfill your request.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipment, please notify us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
- In vivo expression of B56alpha suppressed parasympathetic regulation of heart rate and increased RyR2 phosphorylation in cardiomyocytes. PMID: 26198358
- Cardiomyocyte-directed overexpression of B56alpha, the primary cardiac PP2A regulatory subunit, results in the dephosphorylation of myofilament proteins, enhanced Ca2+ sensitivity, and increased contractility. PMID: 25320082
- Reversible phosphorylation of Dzip1, regulated by the antagonistic actions of CK2 and B56-containing PP2As, has a significant impact on the stability of Gli transcription factors and Hh signaling. PMID: 21878643
- Elevated B56alpha/PP2A inhibits hormone-sensitive lipase and lipolysis in white adipose tissue of DIO mice. PMID: 20534721
- B56alpha plays a role in nuclear export of the catalytic subunit of PP2A PMID: 20378546
- Protein phosphatase 2A contributes to the cardiac dysfunction induced by endotoxemia. PMID: 19201758
