Recombinant Mouse SLIT and NTRK-like protein 2 (Slitrk2), partial

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Description

Research Applications

Recombinant Mouse Slitrk2 (partial) is widely used in:

  • Synapse Formation Assays: Validates Slitrk2’s role in excitatory synapse development via co-culture systems with neurons .

  • Protein Interaction Studies: Identifies binding partners like PTPσ and PDZ-domain scaffolds (e.g., PSD-95, Shank3) .

  • Antibody Production: Serves as an immunogen for generating antibodies targeting Slitrk2’s extracellular region .

  • Neurological Disease Models: Tests functional consequences of Slitrk2 variants observed in neuropsychiatric disorders .

Table 1: Functional Studies Using Recombinant Slitrk2

StudyKey OutcomeCitation
Synaptogenic ActivitySlitrk2-PTPσ interaction enhances excitatory synapse density in hippocampal neurons
Neurite Outgrowth SuppressionRecombinant Slitrk2 inhibits neurite extension in cultured cortical neurons
Pathogenic Variant AnalysisMissense mutations (e.g., V89M) impair synaptic maintenance and TrkB signaling
Structural MappingLRR domains are essential for binding to PTPσ and PDZ scaffolds

Production and Quality Control

  • Expression System: Commonly produced in mammalian cells (e.g., HEK293) to ensure proper glycosylation and folding .

  • Validation: Confirmed via Western blot (WB), immunohistochemistry (IHC), and functional assays in neuronal cultures .

  • Carrier-Free Option: Available for applications where bovine serum albumin (BSA) might interfere .

Future Directions

Current research focuses on:

  • Developing Slitrk2-targeted therapeutics for neurodevelopmental disorders.

  • Elucidating its role in TrkB receptor trafficking and synaptic plasticity .

  • Structural resolution of Slitrk2-PTPσ complexes to guide drug design .

Product Specs

Form
Lyophilized powder
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate it in your order notes. We will accommodate your request whenever possible.
Lead Time
Delivery time may vary depending on the purchasing method and location. Please consult your local distributor for specific delivery time information.
Note: All of our proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please inform us in advance as additional fees will apply.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging the vial briefly before opening to ensure the contents settle at the bottom. Please reconstitute the protein in deionized sterile water to a concentration between 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%. Customers can use this as a reference.
Shelf Life
Shelf life is influenced by various factors, including storage conditions, buffer composition, temperature, and the inherent stability of the protein.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is necessary for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type will be determined during the manufacturing process.
The specific tag type will be determined during production. If you have a specific tag type preference, please inform us, and we will prioritize its development.
Synonyms
Slitrk2; SLIT and NTRK-like protein 2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Slitrk2
Uniprot No.

Target Background

Function
Slitrk2 plays a role in synaptogenesis. It promotes excitatory synapse differentiation and suppresses neurite outgrowth.
Database Links
Protein Families
SLITRK family
Subcellular Location
Membrane; Single-pass type I membrane protein. Cell membrane.
Tissue Specificity
In the adult, significant expression is detected only in the brain. Broadly expressed in embryonic brain with highest expression in ventricular layer, subventricular zone, cortical plate, pyramidal layer of hippocampus, subicular neuroepithelium, thalamus

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