Recombinant Mouse Sphingomyelin synthase-related protein 1 (Samd8)
Description
Introduction to Recombinant Mouse Sphingomyelin Synthase-Related Protein 1 (Samd8)
Recombinant Mouse Sphingomyelin Synthase-Related Protein 1, encoded by the SAMD8 gene, is a protein that has garnered significant attention due to its unique enzymatic activities and roles within cellular processes. Initially identified as a sphingomyelin synthase-related protein, it was later found to possess distinct functionalities, including ceramide phosphoethanolamine synthase activity and phosphatidylethanolamine phospholipase C (PE-PLC) activity .
Phosphatidylethanolamine Phospholipase C (PE-PLC) Activity
Recent studies have revealed that SAMD8 acts as a specific PE-PLC, capable of hydrolyzing phosphatidylethanolamine to produce diacylglycerol (DAG) without requiring ceramide as a substrate. This activity is highly specific, as SAMD8 does not exhibit phospholipase C activity towards other phospholipids like phosphatidylcholine, phosphatidylserine, or phosphatidylglycerol .
Endoplasmic Reticulum Ceramide Regulation
SAMD8 is an endoplasmic reticulum (ER)-resident protein that plays a crucial role in regulating ER ceramide levels. It helps in maintaining ceramide homeostasis, which is essential for preventing ceramide-induced apoptosis .
Expression and Localization
SAMD8 is ubiquitously expressed across various mammalian tissues, although its functional significance varies depending on the tissue type .
Mutational Studies
Mutational analysis of the SAM domain in SAMD8 has highlighted its importance in oligomer formation. Mutations in conserved residues disrupt this oligomerization, affecting the protein's distribution and function .
Table 1: Enzymatic Activities of SAMD8
| Enzymatic Activity | Substrate | Product |
|---|---|---|
| Ceramide Phosphoethanolamine Synthase | Ceramide | Ceramide Phosphoethanolamine (CPE) |
| Phosphatidylethanolamine Phospholipase C (PE-PLC) | Phosphatidylethanolamine | Diacylglycerol (DAG) |
Table 2: Comparison of SAMD8 with DGKδ
| Feature | SAMD8 | DGKδ |
|---|---|---|
| SAM Domain Function | Oligomerization, Subcellular Distribution | Oligomerization, Subcellular Distribution |
| Enzymatic Activity | PE-PLC, CPE Synthase | Diacylglycerol Kinase |
References Nature article on SMSr/SAMD8 as an ER-resident ceramide phosphoethanolamine synthase. Science.gov topic on ceramide metabolism. PMC article on macrophage characterization. PubMed article on SMSr as a mammalian PE-PLC. NCBI gene details for SAMD8. GeneCards information on SAMD8. UniProt entry for SAMD8. LOVD database for SAMD8 variants.
Product Specs
Note: While we prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your preference in the order notes section, and we will prepare the product accordingly.
Note: All proteins are shipped with standard blue ice packs unless otherwise specified. If you require dry ice shipping, please inform us in advance, as additional charges will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C, while lyophilized forms can be stored for 12 months at -20°C/-80°C.
Target Background
- A study revealed that all mouse SMS family members (SMSr, SMS1, and SMS2) possess CPE synthase activity. PMID: 25605874
