Recombinant Mouse TBC1 domain family member 4 (Tbc1d4), partial

Note: We will prioritize shipment of the format currently in stock. If you require a specific format, please specify this in your order notes; we will accommodate your request to the best of our ability.

Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.

This protein may function as a GTPase-activating protein for RAB2A, RAB8A, RAB10, and RAB14. It facilitates insulin-induced translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, thereby increasing glucose uptake.

1. FKBP5 knockout mice exhibit protection against high-fat diet-induced weight gain, improved glucose tolerance, and enhanced insulin signaling in skeletal muscle. A novel association between FKBP51 and AS160 (an AKT2 substrate involved in glucose uptake) has been identified. FKBP51 antagonism increases AS160 phosphorylation. PMID: 29170369
2. AS160 regulates glucose-independent eukaryotic cell proliferation via p21-dependent cell cycle control. PMID: 27152871
3. Studies demonstrate that AS160's RabGAP activity maintains GLUT4 protein levels in a cell/tissue-autonomous manner; its inactivation leads to lysosomal degradation of GLUT4 and postprandial hyperglycemia and hyperinsulinemia. PMID: 27554475
4. AS160 knockout mice exhibit GLUT4 expression and glucose uptake defects in skeletal muscle and adipose tissue. These defects are compounded in AS160 KO/Tbc1d1 KO mice, supporting non-redundant functions for AS160 and Tbc1d1. PMID: 26625902
5. AS160 directly links Na(+),K(+)-ATPase trafficking to the energy state of renal epithelial cells. PMID: 25788531
6. TBC1D4 is not essential for renal Na+ and water transport regulation but may contribute to GLUT4-mediated basolateral glucose uptake in distal tubules. PMID: 26336159
7. AS160 Thr649 phosphorylation by protein kinase B plays a crucial role in the cardiac electrical conduction system by regulating R-wave amplitude. PMID: 25923736
8. AS160 forms a ternary complex with Fsp27 and Rab8a to positively regulate lipid droplet fusion. PMID: 25158853
9. RAB10 and its GTPase-activating protein AS160 constitute the primary signaling module downstream of insulin receptor activation, regulating GLUT4 transport vesicle accumulation at the plasma membrane. PMID: 23804653
10. AS160 knockout mice exhibited insulin resistance in muscle and liver in a euglycemic clamp study. PMID: 23078342
11. Constitutive plasma membrane targeting of AS160 increased surface GLUT4 levels, attributed to enhanced AS160 phosphorylation, 14-3-3 binding, and inhibition of AS160 GAP activity. PMID: 23045393
12. TBC1D4 plays a significant role in regulating renal ion and water handling and extracellular fluid homeostasis. PMID: 22466139
13. In situ contraction or AICAR stimulation slightly decreased both PAS and Thr649 phosphorylation of immunoprecipitated AS160 in various mouse skeletal muscle types. PMID: 22318952
14. AS160 and Rab8a regulate CD36 membrane recruitment. PMID: 22315395
15. Leptin enhances intracellular GLUT4 transport in skeletal muscle of *ob/ob* animals by reducing the expression and activity of GLUT4 traffic negative regulators TBC1D1 and TBC1D4. PMID: 22253718
16. Loss of AS160 causes GLUT4 protein accumulation in compartments primed for fusion in basal adipocytes. PMID: 21613213
17. FABP3 stimulates glucose uptake by facilitating AMPK-dependent AS160 phosphorylation in skeletal muscle. PMID: 21501347
18. Decreased AS160 expression likely contributes to aquaporin-2 translocation to the plasma membrane. PMID: 21511697
19. Results indicate that insulin-induced AS160-Thr649 phosphorylation and/or its 14-3-3 binding play a crucial role in regulating whole-body glucose homeostasis. PMID: 21195350
20. AS160 stabilizes ENaC in a regulated intracellular compartment under basal conditions; aldosterone/SGK1-dependent AS160 phosphorylation enables ENaC forward trafficking to the apical membrane, enhancing sodium absorption. PMID: 20410134
21. S711 is a novel TBC1D4 phosphorylation site regulated by AMPK in skeletal muscle. PMID: 19923418
22. AS160 was identified as an Akt substrate; mass spectrometry and mutation identified two phosphorylation sites, both in RXRXXS/T motifs. PMID: 11994271
23. AS160 plays a role in insulin-regulated GLUT4 trafficking. PMID: 16154996
24. AS160 localizes to GLUT4-containing vesicles via its interaction with IRAP, inhibiting Rab substrate activity and effectively tethering the vesicles intracellularly. PMID: 16762977
25. AS160 provides a mechanism linking AMP kinase signaling to glucose uptake. PMID: 16804075
26. Insulin, contraction, and the AMPK activator AICAR all increased AS160 phosphorylation in mouse skeletal muscle. PMID: 16804077
27. Insulin-dependent 14-3-3 association with AS160 plays a significant role in GLUT4 trafficking in adipocytes. PMID: 16880201
28. AS160 is a critical convergence factor for independent stimulators of skeletal muscle glucose uptake. PMID: 16935857
29. Rab10 is an AS160 target and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation. PMID: 17403373
30. The AS160 calmodulin-binding domain regulates contraction-induced glucose uptake in mouse muscle; calmodulin provides an additional mechanism for modulating AS160 protein. PMID: 17717281
31. The GLUT4 FQQI motif is essential for GLUT4 endocytosis and AS160-dependent intracellular retention but not for GGA-dependent GLUT4 sorting into the insulin-responsive storage compartment. PMID: 17761952
32. Studies demonstrate proportional inhibition of both docking and fusion of GLUT4 storage vesicles by a dominant-negative AS160 mutant. PMID: 18063571
33. The constitutively active Rab10 Q68L mutant is an AS160 GAP domain substrate, hindering rigorous deduction of Rab10 function in its GTP form. PMID: 18076383
34. AS160, known for its role in insulin signaling in skeletal muscle and adipose tissue, is also a major effector of protein kinase B/Akt signaling in beta-cells. PMID: 18276765
35. GLUT4 vesicle recruitment and fusion are differentially regulated by Rac, AS160, and Rab8A in muscle cells. PMID: 18650435
36. TBC1D4 impedes GLUT4 translocation by inactivating a Rab protein on GLUT4-containing membranes. PMID: 18801932
37. RUVBL2 plays a crucial role in insulin-stimulated GLUT4 translocation via its interaction with AS160. PMID: 19532121

KEGG: mmu:210789

STRING: 10090.ENSMUSP00000125509

UniGene: Mm.320639