Recombinant Mouse Transmembrane protein 158 (Tmem158)
Description
Production Data:
| Parameter | Details |
|---|---|
| Expression System | E. coli |
| Tag | N-terminal 10xHis |
| Purity | >95% (SDS-PAGE verified) |
| Storage | -20°C (short-term); -80°C (long-term); avoid freeze-thaw cycles |
Research Applications
Recombinant Mouse Tmem158 is widely used to investigate its roles in:
A. Cancer Biology
-
Glioma: Silencing Tmem158 inhibits glioma cell proliferation and migration by suppressing STAT3 activation .
-
Ovarian Cancer: Overexpression correlates with increased tumor adhesion and invasion via TGF-β1 and BMP4 pathways .
-
Pancreatic Cancer: Linked to larger tumor size and poor prognosis; knockdown reduces metastasis in vivo .
B. Cellular Senescence
-
Acts as a downstream effector of Ras pathway activation, triggering irreversible proliferation arrest .
C. Biomarker Development
-
Used in ELISA kits (e.g., MOEB0832) to quantify Tmem158 levels in biological fluids, with a detection range of 0.156–10 ng/mL and sensitivity of 0.081 ng/mL .
Functional Assays and Tools
-
Therapeutic Target: TMEM158 drives epithelial-mesenchymal transition (EMT) in cancers by activating TGF-β and PI3K/AKT pathways .
-
Prognostic Marker: High expression correlates with poor survival in glioblastoma, ovarian cancer, and laryngeal carcinoma .
Challenges and Future Directions
While recombinant Tmem158 has advanced mechanistic studies, limitations include:
-
Lack of crystal structure data for targeted drug design.
-
Species-specific functional differences between mouse and human orthologs .
Ongoing research focuses on developing TMEM158 inhibitors and exploring its role in modulating immune checkpoints like CTLA4 and LAG3 .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Target Background
Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.
UniGene: Mm.8569
