Recombinant Mouse Tyrosine-protein kinase Mer (Mertk), partial
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Description
Introduction to Recombinant Mouse Tyrosine-protein kinase Mer (Mertk), partial
Recombinant Mouse Tyrosine-protein kinase Mer (Mertk), partial, is a genetically engineered protein derived from the mouse Mertk gene. This protein is produced in mammalian cells and is designed to mimic the function of the native Mer tyrosine kinase, which plays a crucial role in various physiological processes, including the clearance of apoptotic cells and regulation of immune responses .
Structure and Function
The Mertk protein belongs to the Tyro3/Axl/Mer (TAM) family of receptor tyrosine kinases. It consists of two immunoglobulin-like domains, two fibronectin type-III domains, a transmembrane domain, and a cytoplasmic kinase domain . The recombinant version of this protein is expressed with a C-terminal 10xHis tag for purification purposes and includes amino acids 19-497 of the mouse Mertk sequence .
| Feature | Description |
|---|---|
| Protein Type | Recombinant Tyrosine-protein kinase Mer (Mertk) |
| Expression System | Mammalian cells |
| Purity | Greater than 95% by SDS-PAGE |
| Tag | C-terminal 10xHis tag |
| Amino Acid Range | 19-497 |
Biological Role of Mer Tyrosine Kinase
The Mer tyrosine kinase is involved in several biological processes:
-
Apoptotic Cell Clearance: Mer plays a critical role in the recognition and engulfment of apoptotic cells by macrophages, contributing to the resolution of inflammation .
-
Immune Regulation: It acts as an innate immune checkpoint, influencing macrophage polarization and cytokine production .
-
Cell Signaling: Mer signaling affects various pathways, including PI3K/Akt and MAPK/ERK, impacting cell survival and proliferation .
Research Findings and Applications
Research on recombinant Mertk has focused on its potential therapeutic applications, particularly in cancer treatment. Mertk has been shown to regulate cell apoptosis and proliferation, making it a target for tumor therapy . Additionally, studies have explored its role in modulating immune responses and its potential as a therapeutic target in inflammatory diseases .
| Application | Description |
|---|---|
| Cancer Therapy | Targeting Mertk to modulate apoptosis and proliferation in cancer cells |
| Immune Regulation | Investigating Mertk's role in immune checkpoint modulation and inflammatory disease management |
| Apoptotic Cell Clearance | Studying Mertk's function in resolving inflammation through apoptotic cell removal |
Product Specs
Buffer
For liquid delivery forms, the protein is stored in a Tris/PBS-based buffer containing 5%-50% glycerol. Glycerol content can be customized upon request; please specify your requirements when ordering.
Form
The protein is available in liquid or lyophilized powder form. While we prioritize shipping the format currently in stock, specific requests will be accommodated. Please indicate your preferred format during order placement.
Lead Time
Orders typically ship within 1-3 business days of receipt. Delivery times may vary depending on shipping method and location. Contact your local distributor for precise delivery estimates. Standard shipping includes blue ice packs. Dry ice shipping is available upon request with additional charges; please contact us in advance to arrange.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and the inherent stability of the protein. Liquid formulations generally have a 6-month shelf life at -20°C/-80°C. Lyophilized formulations typically have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Available with N-terminal His-tag or Tag-Free.
Synonyms
Mertk; MerTyrosine-protein kinase Mer; EC 2.7.10.1; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK
Datasheet & Coa
Please contact us to get it.
Protein Length
Extracellular domain
Purity
>85% (SDS-PAGE)
Source
Yeast
Species
Mus musculus (Mouse)
Target Names
Uniprot No.
Target Background
Function
MerTK is a receptor tyrosine kinase that transduces signals from the extracellular matrix to the cytoplasm. It binds to several ligands, including LGALS3, TUB, TULP1, and GAS6, and regulates crucial physiological processes such as cell survival, migration, differentiation, and efferocytosis (phagocytosis of apoptotic cells). Ligand binding triggers autophosphorylation of the intracellular MerTK domain, creating docking sites for downstream signaling molecules. Activation leads to interactions with GRB2 or PLCG2 and subsequent phosphorylation of MAPK1, MAPK2, FAK/PTK2, or RAC1. MerTK signaling is involved in macrophage clearance of apoptotic cells, platelet aggregation, cytoskeletal reorganization, and engulfment. In the retinal pigment epithelium (RPE), it regulates phagocytosis of rod outer segment fragments. Furthermore, MerTK plays a significant role in inhibiting Toll-like receptor (TLR)-mediated innate immune responses by activating STAT1, which induces the production of suppressor of cytokine signaling (SOCS1 and SOCS3).
Gene References Into Functions
- MerTK inhibition impairs retinal phagocytic function. (PMID: 29310530)
- MerTK does not affect *S. aureus* phagocytosis but attenuates inflammation via NF-κB inhibition. (PMID: 28528507)
- Tumor macrophage MerTK expression is a potential therapeutic target to prevent tumor recurrence after radiation therapy. (PMID: 27602953)
- Hypercapnic acidosis regulates MerTK shedding and activity. (PMID: 29286859)
- MerTK regulates germinal center B cell responses and autoimmunity via phagocytic and immunomodulatory functions. (PMID: 29118245)
- Viral infection sensitizes fetal membranes through MerTK inhibition. (PMID: 28916522)
- Monocyte-induced MerTK cleavage contributes to myocardial ischemia-reperfusion injury. (PMID: 28851810)
- MerTK cleavage reduces efferocytosis, promoting plaque necrosis and impaired resolution. (PMID: 28067670)
- MerTK is an intracellular negative regulator of the lipoteichoic acid-stimulated macrophage inflammatory response. (PMID: 27419619)
- Axl and Mer receptors cooperatively regulate renal inflammation. (PMID: 27527599)
- Reduced *Mertk* transcription, not protein structure, reduces apoptotic cell clearance efficiency, contributing to atherosclerosis susceptibility. (PMID: 28473436)
- Axl, Mertk, and Tyro3 receptors are not required for Zika virus entry and infection. (PMID: 28423319)
- MerTK signaling promotes specialized pro-resolving mediator (SPM) biosynthesis via 5-lipoxygenase regulation. (PMID: 27199481)
- Dendritic cell clearance of infected, apoptotic neutrophils and Mer receptor signaling are central to *L. major* immune evasion. (PMID: 26658192)
- Tyro3 gene dosage modulates Mertk-associated retinal degeneration and RPE phagocytosis. (PMID: 26656104)
- Mertk activation synergizes with interferon-β to protect brain microvascular endothelial cells from viral transit. (PMID: 26523970)
- TAM receptors control microglial physiology and are potential therapeutic targets in central nervous system diseases. (PMID: 27049947)
- Mertk deficiency alters microRNA expression in retinal pigment epithelial cells. (PMID: 25604732)
- TAM receptors support neural stem cell survival, proliferation, and differentiation by regulating neurotrophin expression. (PMID: 25514676)
- Mertk deficiency affects phagocytosis, cell shape, and migration. (PMID: 25617898)
- Axl and Mer receptors cooperatively regulate systemic immune tolerance to male germ cell antigens. (PMID: 25403570)
- Enhanced Mer signaling increases LXR abundance and activity to inactivate macrophage inflammatory responses. (PMID: 25714463)
- Nuclear receptor agonists increase MerTK and Axl expression, promoting plaque clearance. (PMID: 25904803)
- Mertk expression is crucial for optimal B-cell antigen presentation and T cell-dependent B cell differentiation. (PMID: 24768065)
- Adiponectin elicits Mer expression and Mer-dependent efferocytosis in macrophages. (PMID: 24942043)
- Optimal TAM signaling requires coincident TAM ligand engagement and phosphatidylserine. (PMID: 25265470)
- MerTK cleavage acutely regulates RPE phagocytosis by limiting POS binding. (PMID: 25538233)
- Inhibition of Gas6 receptor Mer or Gas6 targeting reduces myeloma burden and improves survival. (PMID: 25102945)
- Mer mediates quiescence and chemotherapy resistance and causes CNS infiltration. (PMID: 25428221)
- Axl and Mer receptors have distinct roles as phagocytic receptors. (PMID: 25194421)
- MerTK is essential for pyrenocyte engulfment by central macrophages in erythroblastic islands. (PMID: 24659633)
- Mertk links inflammation resolution and organ function in cardiac wound healing. (PMID: 23836795)
- Apoptotic cell engulfment involves Tim4 binding followed by MerTK-mediated engulfment. (PMID: 24515440)
- Axl and Mer receptor knockout mice exhibit chronic hepatitis. (PMID: 23799121)
- Adult brain neurogenesis is reduced in Tyro3-/-Axl-/-Mertk-/- and Axl-/-Mertk-/- mice. (PMID: 24244024)
- Tyro3, Axl, and Mertk knockout mice exhibit chronic systemic inflammation and autoimmune disorders. (PMID: 23840307)
- MEGF10 and MERTK are crucial for synapse remodeling in the brain. (PMID: 24270812)
- MerTK and MFG-E8 are transiently upregulated after focal brain ischemia. (PMID: 24101459)
- Axl and Mer receptor knockout mice exhibit exacerbated inflammation-associated cancer. (PMID: 23878224)
- MerTK signaling suppresses antitumor immunity through CD8+ T lymphocyte proliferation suppression. (PMID: 23867499)
- Mer deficiency leads to long-term apoptotic cell accumulation in germinal centers. (PMID: 23319738)
- Protein S and Gas6 are interchangeable Mer ligands for retinal phagocytosis. (PMID: 23259948)
- Mer mediates HGF production via a RhoA-dependent pathway. (PMID: 22740630)
- Tubby facilitates microglial phagocytosis via MerTK for CNS homeostasis. (PMID: 22884297)
- Mer receptor tyrosine kinase inhibition suppresses STAT1, SOCS1/3, and NF-κB activation, enhancing inflammatory responses in acute lung injury. (PMID: 22427680)
- TAM RTKs regulate male fertility. (PMID: 19602523)
- TRIF signaling activates ADAM17-mediated MerTK proteolysis. (PMID: 21828049)
- TNF family members protect photoreceptors in Mertk(nmf12) homozygotes. (PMID: 21436282)
- Mer(kd) mice exhibit inner retinal dysfunction and faster degeneration. (PMID: 21467171)
- Disrupted Mertk expression enhances marginal zone B-cell responses. (PMID: 20822883)
Database Links
Protein Families
Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily
Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets.
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