Recombinant Mouse Uncharacterized protein C3orf33 homolog
Description
Gene and Protein Characteristics
The C3orf33 gene is located on chromosome 3 in humans (orthologous in mice) and encodes a protein with predicted roles in:
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Negative regulation of ERK1/ERK2 cascade (mitogen-activated protein kinase pathways) .
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Regulation of DNA-binding transcription factor activity, potentially modulating gene expression .
Research Applications and Functional Insights
While the protein’s exact biological role remains uncharacterized, studies suggest its involvement in:
Transcriptional Regulation
Isoform 2 of C3orf33 is secreted and may influence transcription via the MAPK3/MAPK1 pathway, interacting with unidentified plasma membrane receptors . This aligns with its potential role in modulating cellular responses to external signals.
Experimental Tools
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Antibodies: Polyclonal antibodies (e.g., PA5-58047 from Thermo Fisher) target the immunogen sequence
RLTSKFTSSSDIPVEFIRRNVKLRGRLRRIand show 83% cross-reactivity with mouse orthologs . -
Recombinant Proteins: Used in binding assays, co-immunoprecipitation (co-IP), or pull-down experiments to study interactions with other proteins or DNA .
Orthologous Relationships
The protein exhibits sequence conservation across species, as outlined in Table 2.
| Species | Gene | Chromosome | AA Sequence Identity |
|---|---|---|---|
| Human | C3orf33 | 3q22.3 | – |
| Mouse | C3orf33 | – | 83% (with human) |
| Rat | C3orf33 | – | 81% (with human) |
Functional Pathways and Interactions
Limited data exist on direct interactions, but inferred pathways include:
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ERK Signaling Suppression: Regulation of ERK1/ERK2 activity, which impacts cell proliferation and differentiation .
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Transcription Factor Modulation: Potential interaction with DNA-binding proteins to alter gene expression profiles .
Challenges and Future Directions
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Functional Uncertainty: The protein’s role remains speculative due to limited experimental validation.
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Research Gaps: Pathway-specific interactions (e.g., MAPK, transcription factors) require further elucidation.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes if necessary. We will fulfill requests whenever possible.
Note: All proteins are shipped on blue ice unless dry ice shipping is specifically requested and pre-arranged. Additional fees apply for dry ice shipping.
The specific tag will be determined during the production process. If you require a specific tag, please inform us; we will prioritize fulfilling your request.
Target Background
KEGG: mmu:329659
UniGene: Mm.39342
