Recombinant Mouse Uncharacterized protein KIAA0564 homolog (Kiaa0564), partial
Description
Introduction
The protein KIAA0564, also known as Von Willebrand Domain-containing Protein 8 (VWA8), was initially identified through the Kazusa cDNA project . The function of VWA8 was largely unknown, which prompted research into its tissue distribution, cellular location, and function .
Basic Information
Structure and Function
Sequence analysis indicates that VWA8 contains a mitochondrial targeting sequence and domains responsible for ATPase activity . Human and mouse VWA8 have two isoforms: VWA8a and VWA8b. VWA8a consists of 1905 amino acids in both human and mouse, while VWA8b consists of 1039 and 1308 amino acids in human and mouse, respectively . The long and short isoforms in human and mouse share an identical amino acid sequence in the first 1039 (human) or 1038 (mouse) amino acids . The shorter isoforms lack the remaining amino acids due to extensions in Exon 26 that introduce a stop codon (TAA) in both species .
VWA8 has an AAA (ATPase) domain in the N-terminus, which includes Walker A and Walker B motifs . The Walker A motif (GXXGXGK[S/T]) forms a loop that binds to the alpha and beta phosphate moieties of the bound nucleotide, while the Walker B motif, which contains 4 aliphatic residues followed by 2 negatively charged residues, is important for interacting with $$Mg^{2+}$$ . Deletion of either the Walker A or Walker B motif largely blocked ATPase activity, which suggests that these motifs are essential for VWA8's ATPase activity .
Homology Modeling
Homology modeling, using the Phyre2 server, revealed that 52% of the VWA8a sequence could be modeled with 100% confidence based on the structure of the cytoplasmic dynein heavy chain (PDB c3ykhB) . VWA8a and the dynein heavy chain motor domain have 16% identity at the amino acid level . Furthermore, 90% of the VWA8b sequence could be modeled with 100% confidence using the cytoplasmic dynein heavy chain (c3vkhb) as a template . These models indicate structural similarities to dynein-related motor proteins .
Table 1: Homology Modeling Results
| Protein | Template Protein | Percentage of Sequence Modeled | Confidence | Amino Acid Identity |
|---|---|---|---|---|
| VWA8a | Cytoplasmic dynein heavy chain | 52% | 100% | 16% |
| VWA8b | Cytoplasmic dynein heavy chain | 90% | 100% | N/A |
Product Specs
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Target Background
- Analyses reveal that the mouse Vwa8 gene produces two transcripts: the full-length Vwa8a, which is highly expressed compared to the truncated Vwa8b across all developmental stages and tissues examined. This study establishes the developmental timeline and expression profile for both Vwa8a and Vwa8b. PMID: 29660410
- In mouse AML12 liver cells, VWA8 protein localized exclusively to mitochondria; this localization was prevented by deleting the targeting sequence. PMID: 28414126
