Recombinant Mouse Vasohibin-1 (Vash1)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing.
If a specific tag type is required, please inform us; we will prioritize its implementation.
If a specific tag type is required, please inform us; we will prioritize its implementation.
Synonyms
Vash1; Vash; Tubulinyl-Tyr carboxypeptidase 1; EC 3.4.17.17; Tyrosine carboxypeptidase 1; TTCP 1; Vasohibin-1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-375
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mus musculus (Mouse)
Target Names
Vash1
Target Protein Sequence
MPGGKKVVPS GSSSASPNAA ATTTAAAAAA AAAPHSGTKR LETTEGASAQ RDEEPEEEGE
EDLRDGGVPF FINRGGLPVD EATWERMWKH VAKIHPDGEK VALRIRGATD LPKIPIPSVP
TFQPTTPVPE RLEAVQRYIR ELQYNHTGTQ FFEIKKSRPL TGLMDLAKEM TKEALPIKCL
EAVILGIYLT NSMPTLERFP ISFKTYFSGN YFRHIVLGVN FGGRYGALGM SRREDLMYKP
PAFRTLSELV LDYEAAYGRC WHVLKKVKLG QCVSHDPHSV EQIEWKHSVL DVERLGREDF
RKELERHARD MRLKIGKGTG PPSPTKDRKK DVSSPQRAQS SPHRRNSRSE RRPSGEKKPA
EPKAMPDLSG YQIRV
Uniprot No.
Target Background
Function
Vasohibin-1 (VASH1) is a tyrosine carboxypeptidase that removes the C-terminal tyrosine residue from α-tubulin, thereby modulating microtubule dynamics and function. It acts as an angiogenesis inhibitor, suppressing endothelial cell migration, proliferation, and network formation, thereby inhibiting angiogenesis. This inhibitory effect is specific to endothelial cells, as it does not affect smooth muscle cell or fibroblast migration.
Gene References Into Functions
- In replicative senescence, downregulation of VASH1 expression in endothelial cells is partly attributed to altered microRNA expression. PMID: 27325558
- Cancer cells proteolytically inactivate VASH1 secreted by endothelial cells within the tumor microenvironment. PMID: 27169581
- Vasohibin-1 knockout resulted in unaffected fibrosis and angiogenesis. PMID: 26025651
- Endogenous VASH1 may regulate the development of diabetic renal alterations. PMID: 25255225
- PKCδ deficiency enhances neointimal formation, correlating with delayed re-endothelialization and increased cellular vasohibin-1 accumulation. PMID: 20884873
- Vasohibin-1 is the first known intrinsic factor with broad-spectrum anti-lymphangiogenic activity. PMID: 20133819
- Vasohibin acts as a negative feedback regulator of retinal neovascularization; suppression of VEGF receptor 2 may contribute to its activity. PMID: 16473886
- Vasohibin prevents neointimal formation by inhibiting adventitial angiogenesis. PMID: 16707096
- VASH1 plays a role in the negative feedback regulation of hematopoietic progenitor proliferation during bone marrow recovery. PMID: 19179360
- Loss of vasohibin-1 is associated with persistent angiogenesis in the endothelial cell termination zone. PMID: 19204325
- Endogenous vasohibin-1 is involved in tumor angiogenesis; exogenous vasohibin-1 blocks sprouting angiogenesis, matures vessels, and enhances the antitumor effect of chemotherapy. PMID: 19498005
- Murine VASH1 overexpression inhibited angiogenic sprouting and promoted vascular maturation in vivo. PMID: 19682397
Database Links
Protein Families
Vasohibin family
Subcellular Location
Cytoplasm. Secreted.
Tissue Specificity
Expressed at low level in proliferating endothelial cells at the sprouting front but highly expressed in nonproliferating endothelial cells in the termination zone.
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