Recombinant Mouse VIP36-like protein (Lman2l)
Description
Production and Purification
Recombinant Mouse VIP36-like protein is synthesized using multiple expression systems:
| Host System | Purity | Tag | Applications |
|---|---|---|---|
| E. coli | ≥85% | None/His-tag | SDS-PAGE, WB, ELISA |
| Yeast/Baculovirus | ≥85% | Variable | Structural studies |
| Mammalian Cells | >95% | His-tag | Functional assays, immunoprecipitation |
Glycoprotein Trafficking:
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Regulates ER export of select glycoproteins by interacting with ERGIC-53, a cargo receptor .
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Loss of LMAN2L disrupts surface expression of integrin α6 (ITGA6), suggesting a role in vesicular transport .
Viral Interactions:
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Human cytomegalovirus (HCMV) protein US2 targets LMAN2L for TRC8-mediated degradation to impair host immune responses .
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Associates with enterovirus 3A protein, SCAMP3, and PI4KIIIβ to form replication complexes, enhancing viral RNA synthesis .
Sugar-Binding Activity:
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Demonstrates calcium-dependent lectin activity for high-mannose glycans, critical for quality control in the ER .
Table 1: Select Studies on Recombinant Mouse VIP36-like Protein
Research Applications
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Glycobiology: Used to analyze mannose-binding specificity and ER-Golgi transport mechanisms .
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Virology: Employed in studies on enterovirus and HCMV replication strategies .
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Protein Interaction Mapping: Facilitates identification of binding partners (e.g., SCAMP3, PI4KIIIβ) via co-immunoprecipitation .
Challenges and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it during order placement, and we will fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If dry ice shipping is required, please contact us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
