Recombinant Mus caroli Nuclear autoantigen Sp-100 (Sp100)

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Cat. No.

BT33099

Source

Yeast

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33099

Source

E.coli

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33099

Source

E.coli

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33099

Source

Baculovirus

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33099

Source

Mammalian cell

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant Mus caroli Sp100

Sp100 is a nuclear antigen localized in subnuclear domains called nuclear dots (NDs) or PML bodies. The Mus caroli variant shares structural and functional similarities with its human homolog, including interferon responsiveness and involvement in autoimmune responses . Recombinant production enables study of its biochemical interactions and diagnostic applications.

Gene and Protein Features

Gene Structure: The Mus caroli Sp100 gene spans 52 kb with 17 exons. Its promoter contains an interferon-stimulated response element (ISRE), critical for cytokine-induced expression .
Isoforms: Splice variants include HNPP-box and HMG1-containing forms, influencing nuclear localization and protein interactions .
Molecular Weight:
- Calculated MW: ~54 kDa (human Sp100-A isoform)
- Apparent MW: 70–140 kDa (aberrant electrophoretic mobility due to acidic residues)

Expression Systems and Purification

Parameter	Details
Host Systems	Sf9 insect cells , Escherichia coli
Tag	Hexa-histidine (His-tag) or GST tag
Purity	>80% (SDS-PAGE) ; >95% (E. coli-derived)
Storage	-20°C in HEPES/NaCl/glycerol buffer ; lyophilized with trehalose/DTT

Key Functions

Protein Interactions: Binds HP1 family proteins (e.g., CBX5) via PxVxL motifs and Epstein-Barr virus EBNA-LP .
Autoantibody Binding: Recognized by IgG autoantibodies in PBC patients .
Interferon Response: IFN-α/β/γ upregulates Sp100 expression, enlarging nuclear dots .

Table: Key Studies on Recombinant Sp100

Study Focus	Findings	Source
Interferon Regulation	Murine Sp100 expression induced by IFN via ISRE; conserved in humans
Clinical Diagnostics	Anti-Sp100 antibodies detected in 34% of PBC patients and 13% of SLE cases
Antibody Applications	Recombinant Sp100 used in ELISA/Western blot with 1:2000–1:10,000 dilution

Clinical and Diagnostic Relevance

Primary Biliary Cirrhosis: Anti-Sp100 antibodies present in 10–30% of PBC cases but lack disease specificity .
Limitations: Also found in SLE, hepatitis, and collagen diseases, necessitating clinical context for diagnosis .

Applications in Research

Western Blotting: Detected in A431 and HeLa cell lysates at 70–140 kDa .
Immunofluorescence: Localizes to nuclear dots in IFN-treated cells .
ELISA: Coating concentration 0.5–0.9 µg/ml for autoantibody screening .

Product Specs

Form

Lyophilized powder. We will ship the available format, but you can specify your preference when ordering.

Lead Time

Delivery times vary. Consult your local distributor for specifics. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. Specify your preferred tag type during ordering.

Synonyms

Sp100; Nuclear autoantigen Sp-100; Nuclear dot-associated Sp100 protein; Speckled 100 kDa

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-482

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Mus caroli (Ryukyu mouse) (Ricefield mouse)

Target Names

Sp100

Target Protein Sequence

MEDSNASPRM STEHENTEMH PFEYMFKHFK TQKVAISNAI RSTFPFLESL RDREFITGKM YEDLIDSCRS LVPVDKVIYK ALDELEKKFD VTVLWELFNE VNMEKYPDLN PIRRSFECVF PNELSFQGID RGNPNSQLSL EQGPSASYSQ GSLNGSSLDL SSSEGWRSND RRNSNLMQAN QTENHQLAES PGHLDSCELQ VQLNNGDATP ESYSLLPQHE ERAVQLNNEF QINPCFVQLI DVKKENSSFS LAGNQQTRAR TNQNEDSEVI ELSSGDSDDG ENFSEATTTI PSQPAPAYSR TPPTLRTDRR GDTSDTESSI IIRRRKRTGR KKRERLGSYL IRNIKIPMKT SWKTAVLARS ANTSSQRRRK RGPRIPREEN ADFGGAELPA VCGNVQGFLN KEKFKQGIYV RSIRSETGRL FTPMDFEIEG NCEKAKNWRQ TIRCKGWTLR ELIQKGVLQD PPRKKKENPR NPRQMKRQVN AL

Uniprot No.

O35893

Target Background

Function

Sp100, along with PML, is a major component of PML bodies, involved in cell growth, differentiation, and apoptosis. It acts as a transcriptional coactivator/corepressor for ETS1/ETS2, influencing angiogenesis. It may also regulate telomere lengthening, TP53-mediated transcription, FAS-mediated apoptosis, and viral infection through chromatin/transcriptional regulation.

Subcellular Location

Nucleus. Nucleus, PML body. Cytoplasm.

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Recombinant Mus caroli Nuclear autoantigen Sp-100 (Sp100)

Description

Introduction to Recombinant Mus caroli Sp100

Gene and Protein Features

Expression Systems and Purification

Key Functions

Table: Key Studies on Recombinant Sp100

Clinical and Diagnostic Relevance

Applications in Research

Product Specs

Target Background

Quick Inquiry

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