Recombinant Mycobacterium bovis Fumarate reductase subunit D (frdD)
Description
Introduction to Recombinant Mycobacterium bovis Fumarate Reductase Subunit D (frdD)
Recombinant Mycobacterium bovis Fumarate reductase subunit D (frdD) is a protein produced through recombinant DNA technology. This protein is part of the fumarate reductase complex, which plays a crucial role in the metabolic pathways of mycobacteria. The fumarate reductase complex is involved in the electron transport chain and is essential for the survival of these bacteria under anaerobic conditions.
Production and Characteristics
The recombinant frdD protein is typically produced in an Escherichia coli expression system, which allows for high purity and yield. This method involves cloning the gene encoding frdD into a plasmid, followed by transformation into E. coli cells, where the protein is expressed and purified .
Biological Significance:
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Metabolic Pathways: Essential for anaerobic respiration and energy production.
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Drug Target: The fumarate reductase complex, including frdD, is considered a potential drug target due to its role in mycobacterial metabolism .
Research Findings and Applications
Research on the fumarate reductase complex, including frdD, has focused on understanding its metabolic role and potential as a drug target. Studies have shown that this complex may have evolved to perform additional functions beyond its primary metabolic role, particularly in the interaction between mycobacteria and their host cells .
Research Highlights:
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Evolutionary Adaptations: The fumarate reductase operon has evolved differently in M. bovis compared to M. tuberculosis, suggesting adaptive responses to environmental pressures .
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Drug Development: The complex is a potential target for drug design, although its redundancy with other metabolic pathways complicates this approach .
Product Specs
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Target Background
KEGG: mbt:JTY_1581
