Recombinant Mycobacterium smegmatis Glycine cleavage system H protein (gcvH)

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Cat. No.
BT35746
Source
Yeast
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35746
Source
E.coli
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35746
Source
E.coli
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35746
Source
Baculovirus
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT35746
Source
Mammalian cell
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Description

Introduction to the Glycine Cleavage System H Protein (GcvH)

The glycine cleavage system (GCS) is a multienzyme complex critical for glycine metabolism, functioning in one-carbon unit transfer and folate metabolism. The H protein (GcvH) is a lipoyl-bearing component that shuttles intermediates between the P and T proteins of the GCS. In Mycobacterium smegmatis, GcvH plays a role in central metabolic pathways, though its recombinant form has not been as extensively studied as in other bacterial species like Bacillus or Escherichia coli .

Production and Purification of Recombinant GcvH

While direct data on M. smegmatis GcvH is limited, homologous systems provide insights:

ParameterExample from Bacillus cereus GcvH Inferred for M. smegmatis
Host organismYeastLikely E. coli or mycobacterial systems
TagHis-tagHis-tag or mycobacterial tags (e.g., PE_PGRS33)
Purity>90%>85% (based on analogous workflows)
ApplicationsELISA, enzymatic assaysStructural studies, metabolic assays

Steps for recombinant production:

  1. Gene cloning into a mycobacteria-compatible vector (e.g., pMV306 or integrative plasmids) .

  2. Transformation into M. smegmatis mc² 155 or similar strains.

  3. Induction with acetamide or tetracycline .

  4. Purification via affinity chromatography (e.g., Ni-NTA for His-tagged proteins) .

4.1. Metabolic and Proteomic Insights

  • Proteomic studies of M. smegmatis under dormancy or stress highlight shifts in one-carbon metabolism enzymes, though GcvH was not explicitly identified .

  • Homology: M. smegmatis GcvH likely shares functional overlap with Bacillus homologs, given conserved domains (e.g., lipoyl-binding motif) .

4.2. Biotechnological Utility

  • Vaccine development: M. smegmatis is used as a recombinant protein expression host for antigens (e.g., SARS-CoV-2 spike protein) . GcvH could theoretically serve as a carrier or adjuvant, though this remains unexplored.

  • Enzymatic assays: Recombinant GcvH from Bacillus is used in glycine decarboxylation studies; similar applications are feasible for M. smegmatis .

Challenges and Knowledge Gaps

  • Limited direct studies: No publications explicitly describe M. smegmatis GcvH purification or characterization.

  • Lipoylation efficiency: In M. smegmatis, lipoylation machinery differs from E. coli, potentially affecting recombinant GcvH activity .

  • Functional redundancy: M. smegmatis may employ alternative one-carbon pathways under stress, reducing reliance on GCS .

Future Directions

  1. Structural characterization: Cryo-EM or X-ray crystallography of recombinant M. smegmatis GcvH.

  2. Knockout studies: Assess GcvH’s role in glycine metabolism and stress responses.

  3. Biotechnological optimization: Engineer M. smegmatis strains for high-yield GcvH production .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Request dry ice shipping in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
gcvH; MSMEG_3648; MSMEI_3562; Glycine cleavage system H protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-131
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Target Names
gcvH
Target Protein Sequence
MSEIPADLYY TSEHEWVLRT GDDTVRVGIT DYAQSALGDV VFVQLPDVGA DVASGDAFGE VESTKSVSDL YAPVTAKVVA VNGDLEGSPE LVNSDPYGEG WLVDLRVEAG TLDEALGGLL DAEGYRAVVT E
Uniprot No.
A0QYG3

Target Background

Function
The glycine cleavage system breaks down glycine. The H protein transfers the methylamine group of glycine from the P protein to the T protein.
Database Links

KEGG: msb:LJ00_18140

STRING: 246196.MSMEG_3648

Protein Families
GcvH family

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