Recombinant Mycobacterium smegmatis Protein translocase subunit SecA 1 (secA1), partial

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Cat. No.
BT33801
Source
Yeast
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33801
Source
E.coli
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33801
Source
E.coli
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33801
Source
Baculovirus
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33801
Source
Mammalian cell
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Purity
>85% (SDS-PAGE)
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Description

Essentiality of SecA1

Allelic exchange experiments in M. smegmatis revealed that secA1 cannot be deleted unless complemented by a functional copy (Table 1) .

Table 1: Outcomes of secA1 Allelic Exchange in M. smegmatis

Strain (Plasmid)% ΔsecA1 Recombinants% Wild-Type RecombinantsTotal Recombinants (n)
MB526 (no complement)0%100%13
MB526 (M. smegmatis secA1)62%38%16
MB526 (M. tuberculosis secA2)0%100%20

Deletion of ATP-binding domains in a truncated SecA1 (531 aa) rendered the protein nonfunctional, confirming its essential role .

Role in Protein Export

SecA1 drives the translocation of signal sequence-containing proteins, including virulence factors. Key findings include:

  • Azide Sensitivity: ΔsecA2 mutants exhibited hypersensitivity to sodium azide, implicating SecA1 as the primary ATPase .

  • Substrate Recognition: SecA1 exports ~95% of M. smegmatis secreted proteins, including porins like MspA .

  • Interaction with SecA2: Although nonessential, SecA2 assists SecA1 in exporting specific substrates (e.g., esterases), but cannot compensate for SecA1 loss .

Recombinant SecA1 in Pathogenesis Studies

Partial SecA1 constructs have been used to:

  1. Identify Secretion Substrates: Silencing secA1 in M. smegmatis reduced MspA porin levels by >90%, confirming its role in cell envelope biogenesis .

  2. Study Virulence Mechanisms: Recombinant M. smegmatis expressing M. tuberculosis SecA1-dependent proteins (e.g., Rv1954A) showed enhanced macrophage infectivity and apoptosis induction .

Therapeutic Implications

SecA1 is a potential drug target due to its essentiality. Overexpression of SecA1 exacerbates growth defects in ΔsecA2 mutants, suggesting that disrupting SecA1-SecA2 synergy could inhibit virulence . Additionally, SecA1’s conserved ATPase domains make it vulnerable to ATP-competitive inhibitors .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but if you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please contact us in advance, as additional charges will apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C is recommended. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer composition, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
secA1; MSMEG_1881; MSMEI_1840Protein translocase subunit SecA 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Target Names
secA1
Uniprot No.
P71533

Target Background

Function
This protein is part of the Sec protein translocase complex and interacts with the SecYEG preprotein conducting channel. It plays a key role in coupling ATP hydrolysis to protein transfer across the cell membrane, acting as an ATP-driven molecular motor that drives the stepwise translocation of polypeptide chains.
Database Links

KEGG: msb:LJ00_09385

STRING: 246196.MSMEG_1881

Protein Families
SecA family
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm.

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