Recombinant Mycoplasma agalactiae ATP synthase subunit alpha (atpA), partial
Shipped with Ice Packs 
In Stock 
Description
Introduction
The ATP synthase subunit alpha (atpA) is a critical component of the FF ATP synthase complex, responsible for ATP synthesis in prokaryotes. In Mycoplasma agalactiae, a minimal pathogen causing contagious agalactia in ruminants, the ATP synthase plays a key role in energy metabolism despite the organism’s reduced genome and host-dependent lifestyle . Recombinant production of partial atpA enables detailed study of its structural and functional properties, aiding in understanding pathogenicity and metabolic adaptations .
Recombinant Expression and Functional Analysis
Comparative Analysis with Other Mycoplasma Species
Implications for Pathogenicity and Metabolism
-
Metabolic Adaptations:
-
Host Interaction:
Data Tables
Table 1: Proteomic Identification of ATP Synthase Subunits in M. agalactiae
| Protein | gi Accession No. | Fold Change (Infected vs. Control) | P Value |
|---|---|---|---|
| ATP synthase O subunit | gi|57108786 | 1.58 | 0.0212529 |
| Chain A, mitochondrial F ATPase | gi|1943080 | 5.27 | <0.0001 |
Source: Proteomic analysis of milk fat globules during infection
Product Specs
Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
atpA; MAG3540ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Mycoplasma agalactiae (strain PG2)
Target Names
atpA
Uniprot No.
Target Background
Function
Produces ATP from ADP using a proton gradient across the membrane. The alpha chain is a regulatory subunit.
Database Links
KEGG: maa:MAG3540
Protein Families
ATPase alpha/beta chains family
Subcellular Location
Cell membrane; Peripheral membrane protein.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
