Recombinant Mycoplasma genitalium Uncharacterized protein MG220 (MG220)
Description
Production and Expression Systems
MG220 is primarily produced in E. coli using inducible promoter systems (e.g., T7, lac, tac) . Alternative expression hosts include yeast, baculovirus, mammalian cells, and cell-free systems, though E. coli remains the most common due to cost-effectiveness .
Diagnostic and Serological Studies
MG220 is used in ELISA kits for detecting anti-Mycoplasma genitalium antibodies, though its specificity remains under investigation . Unlike MG075 (a validated serological marker for M. genitalium), MG220’s cross-reactivity with Mycoplasma pneumoniae is unconfirmed .
Challenges and Considerations
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Uncharacterized Function: MG220’s biological role in M. genitalium remains undefined, limiting its utility in mechanistic studies.
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Solubility Issues: Hydrophobic regions may necessitate denaturing conditions (e.g., urea) for proper handling .
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Strain Variability: While MG075 exhibits low variability, MG220’s antigenic stability across M. genitalium strains is unverified .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will fulfill your request.
Note: All our proteins are shipped with standard blue ice packs. If dry ice shipment is required, please inform us in advance, and additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: mge:MG_220
