Recombinant Mycoplasma mobile Putative Holliday junction resolvase (MMOB4380)
Description
Definition and Genomic Context
MMOB4380 is a hypothetical protein encoded by the Mycoplasma mobile genome. Based on sequence homology, it is predicted to belong to the Holliday junction resolvase family, which catalyzes the resolution of HJs into recombinant DNA products. HJs form during genetic recombination, requiring enzymatic resolution to prevent chromosomal segregation errors .
Key Characteristics (Hypothetical):
| Feature | Description |
|---|---|
| Gene ID | MMOB4380 |
| Protein Length | Predicted size (not experimentally determined) |
| Homologs | Potential similarity to RecU (M. genitalium) or RuvC (E. coli) |
| Functional Role | Putative HJ resolution via endonucleolytic cleavage |
Holliday Junction Resolvases: General Mechanism
HJ resolvases are structure-selective endonucleases that recognize and cleave HJs with high specificity . Their mechanisms include:
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Symmetric Cleavage: Introducing nicks across two strands of the HJ to yield recombinant duplexes.
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Dimerization: Many resolvases (e.g., RuvC, GEN1) function as dimers, enabling coordinated cleavage .
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Catalytic Residues: Conserved acidic residues (e.g., Asp, Glu) mediate metal ion coordination and cleavage .
Examples in Mycoplasma:
Functional Hypotheses
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Resolution of HJs: MMOB4380 may resolve HJs formed during recombination, ensuring proper chromosome segregation.
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Antigenic Variation: Mycoplasma species use recombination to diversify surface proteins (e.g., Mvsps in M. mobile) . MMOB4380 could facilitate this process.
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DNA Repair: Involvement in repairing double-strand breaks via homologous recombination.
Gaps in Knowledge and Future Directions
| Aspect | Limitations | Research Needs |
|---|---|---|
| Experimental Data | No biochemical assays or crystal structures | Purification, cleavage assays, mutational studies |
| Genomic Context | Unknown regulatory elements or operons | Transcriptomic/proteomic profiling |
| Species-Specificity | Role in M. mobile's pathogenicity | Comparative studies with other Mycoplasma spp. |
Product Specs
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Target Background
KEGG: mmo:MMOB4380
STRING: 267748.MMOB4380
