Recombinant Mycoplasma pneumoniae Putative ABC transporter ATP-binding protein MG014 homolog (MPN_018)
Description
Protein Structure and Sequence
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Gene Locus: MPN_018 (annotated in Mycoplasma pneumoniae strain M129) .
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Amino Acid Sequence: Comprises 623 residues, with a calculated molecular weight of 68.5 kDa . Key motifs include conserved Walker A/B ATP-binding domains and ABC signature sequences .
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Post-Translational Modifications: Acetylation at lysine residue K576 has been reported .
Expression and Purification
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Host System: Expressed in Escherichia coli with an N-terminal His-tag for purification .
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Form: Lyophilized powder in Tris/PBS buffer (pH 8.0) with 6% trehalose .
| Parameter | Details |
|---|---|
| Expression System | E. coli |
| Tag | N-terminal 10×His-tag |
| Storage | -20°C/-80°C; avoid repeated freeze-thaw cycles |
| Reconstitution | 0.1–1.0 mg/mL in sterile water; glycerol (5–50%) recommended for stability |
ABC Transporter Activity
ABC transporters utilize ATP hydrolysis to drive substrate translocation across membranes. MPN_018 is hypothesized to function in:
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Drug Resistance: Homology to eukaryotic multidrug resistance (MDR) proteins suggests a role in antibiotic efflux .
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Metabolite Transport: Potential involvement in nutrient uptake (e.g., phosphate, metals) critical for bacterial survival .
Pathogenicity and Host Interaction
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Adhesion and Virulence: While not a primary adhesin like P1, MPN_018 may contribute to virulence by interacting with host immune components .
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Immune Evasion: ABC transporters in Mycoplasma species degrade neutrophil extracellular traps (NETs), aiding immune evasion .
Antibiotic Resistance Studies
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Macrolide-resistant M. pneumoniae strains (e.g., MRMp) often exhibit mutations in ABC transporters, altering drug-binding affinity .
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Recombinant MPN_018 enables structural studies to map resistance mutations (e.g., 23S rRNA mutations) .
Clinical and Epidemiological Relevance
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Outbreak Surveillance: Genomic recombination hotspots (e.g., MPN366–MPN371) near ABC transporter genes drive strain diversity and antibiotic resistance .
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Diagnostic Targets: MPN_018’s immunogenicity makes it a candidate for serological assays, though less prominent than CARDS toxin .
Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will prepare according to your request.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance. Additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: mpn:MPN018
