Recombinant Mycoplasma pneumoniae Uncharacterized protein MG027 homolog (MPN_030)
Description
Overview of Mycoplasma pneumoniae Protein Homologs
The M. pneumoniae genome contains multiple uncharacterized proteins with homology to other mycoplasma species. While MPN_030 is not mentioned in the provided literature, several related homologs have been studied:
Genomic Context
M. pneumoniae strain M129 contains 688 predicted open reading frames (ORFs) , but MPN_030 is not discussed in any of the provided studies. Proteins like MPN_031 (MG028 homolog) and MPN_311 (MG2181 homolog) are documented in structural and recombinant expression studies, suggesting MPN_030 may belong to a less-characterized protein family.
Potential Reasons for Lack of Information
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Low abundance: Only 56% of M. pneumoniae proteins were detected in N-terminome studies .
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Non-essential function: Uncharacterized proteins often evade detection in targeted assays unless linked to virulence or host interactions.
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Technical limitations: Membrane-associated proteins like MPN_030 may require specialized extraction protocols not employed in the cited studies .
MPN_031 (MG028 Homolog)
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Expression: Recombinant full-length protein (1–203 aa) expressed in E. coli with N-terminal His tag .
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Sequence: Contains conserved domains of unknown function (DUF) but lacks catalytic activity in preliminary assays .
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Applications: Used in SDS-PAGE analysis; storage at -80°C recommended .
MPN_311 (MG2181 Homolog)
Recommendations for Future Research
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Proteomic profiling: Use LC–MS/MS with immunoprecipitation to identify MPN_030 in M. pneumoniae lysates .
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Functional assays: Test recombinant MPN_030 for interactions with host immune receptors (e.g., NOD2, TLRs) using pull-down assays .
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Structural studies: Employ cryo-EM or X-ray crystallography to resolve MPN_030’s tertiary structure, leveraging homology to MG028 or DUF16 .
