Recombinant Nasalis larvatus C-C chemokine receptor type 5 (CCR5)
Description
Functional Significance
CCR5 is a seven-transmembrane GPCR involved in immune cell migration (chemotaxis) and inflammatory responses . Its ligands include chemokines such as CCL3 (MIP-1α), CCL4 (MIP-1β), and CCL5 (RANTES), which regulate T-cell trafficking and macrophage activation . In HIV research, CCR5 serves as a primary co-receptor for viral entry, making it a therapeutic target for inhibitors like maraviroc .
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Key roles:
Research Applications
This recombinant protein is utilized in:
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Structural studies: CCR5’s transmembrane topology and ligand-binding mechanisms are analyzed using cryo-EM and X-ray crystallography . For example, the N-terminal domain and extracellular loops form critical binding interfaces for chemokines like CCL5 .
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HIV inhibition assays: Compounds targeting CCR5 (e.g., AOP-RANTES, NNY-RANTES) are tested for blocking viral entry .
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Cross-species virology: Non-human primate CCR5 variants, including Nasalis larvatus, help study HIV adaptation and zoonotic potential .
Comparative Insights
While human and Nasalis CCR5 share 92% sequence homology, subtle differences in extracellular loops impact ligand binding and viral tropism . For instance:
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The V3 loop of HIV gp120 interacts with CCR5’s extracellular domains, and mutations here alter coreceptor specificity .
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Nasalis CCR5’s conserved disulfide bonds (Cys28–Cys274) stabilize its tertiary structure, similar to human CCR5 .
Technical Considerations
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Storage: Lyophilized protein remains stable at -80°C but degrades upon repeated thawing .
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Activity assays: Functional validation requires reconstitution in lipid bilayers or cell membranes to preserve GPCR conformation .
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Safety: Not for human therapeutic use due to potential immunogenicity .
Future Directions
Research priorities include:
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order, and we will accommodate your request.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
