Recombinant Nasua nasua Hemoglobin subunit alpha (HBA)

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Cat. No.

BT31204

Source

Yeast

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31204

Source

E.coli

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31204

Source

E.coli

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31204

Source

Baculovirus

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31204

Source

Mammalian cell

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant Hemoglobin Subunit Alpha

Recombinant hemoglobin subunit alpha (HBA) is a synthetically produced globin protein critical for oxygen transport in vertebrates. While native hemoglobin is a tetramer of two α- and two β-subunits, recombinant versions allow controlled production for research and therapeutic applications .

Table 1: Comparison of Recombinant HBA Variants

Feature	Abcam (ab158638)	Prospec Bio (PRO-1183)	Bio-Techne (H00003039-Q01)
Expression Host	Wheat germ	E. coli	Wheat germ
Tag	None	N-terminal His-tag (37 aa)	N-terminal GST tag
Purity	>85% (SDS-PAGE)	>90% (SDS-PAGE)	>80% (SDS-PAGE)
Applications	ELISA, WB	Research	Research
Storage	-80°C	-20°C (with glycerol/DTT)	Not specified

Purification Methods:

His-tag affinity chromatography: Used for E. coli-expressed HBA .
SDS-PAGE validation: Confirms purity and molecular weight .

Research Applications

Oxygen Carrier Development: Recombinant HBA serves as a scaffold for engineered hemoglobin-based oxygen carriers (HBOCs) with reduced nitric oxide dioxygenation (NOD) activity .
Mutagenesis Studies: Key residues (e.g., Trp(B10), Gln(E7)) are modified to alter O₂ affinity and stability .
Antibody Validation: Used as a control in western blotting to test specificity of anti-hemoglobin antibodies .

Challenges and Innovations

Tetramer Stability: Genetic crosslinking (e.g., rHb0.1) prevents dimer dissociation under physiological conditions .
Post-Translational Modifications: Variants like Thionville retain the initiator methionine, affecting acetylation .
Heme Incorporation: E. coli systems require exogenous heme for functional tetramer assembly .

Future Directions

Therapeutic HBOCs: Engineering low-NOD variants to mitigate vasoconstrictive side effects .
High-Yield Systems: Optimizing E. coli and wheat germ platforms for industrial-scale production .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will try to accommodate your request.

Lead Time

Delivery time varies depending on purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional fees apply.

Notes

Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form generally lasts 6 months at -20°C/-80°C. Lyophilized form generally lasts 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

HBAHemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-141

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Nasua nasua (Ring-tailed coati)

Target Names

HBA

Target Protein Sequence

VLSPADKTNI KSTWEKIGSH ASEYGGEALE RTFASFPTTK TYFPHFDLSP GSAQVKAHGK KVAEALTNAV AHLDDLPGAL STLSDLHAYK LRVDPVNFKF LSHCLLVTLA SHHPAEFTPA VHASLDKFFS SVSTVLTSKY R

Uniprot No.

P26915

Target Background

Function

Involved in oxygen transport from the lungs to peripheral tissues.

Protein Families

Globin family

Tissue Specificity

Red blood cells.

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Recombinant Nasua nasua Hemoglobin subunit alpha (HBA)

Description

Introduction to Recombinant Hemoglobin Subunit Alpha

Table 1: Comparison of Recombinant HBA Variants

Purification Methods:

Research Applications

Challenges and Innovations

Future Directions

Product Specs

Target Background

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