Recombinant Nautilia profundicola Queuine tRNA-ribosyltransferase (tgt)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will preferentially ship the available format, but will accommodate special format requests if specified when ordering.
Lead Time
Delivery times vary by purchase method and location. Contact your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. Requesting dry ice will incur extra fees and requires advance notice.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form generally lasts 6 months at -20°C/-80°C. Lyophilized form typically lasts 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
tgt; NAMH_0482; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-378
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Nautilia profundicola (strain ATCC BAA-1463 / DSM 18972 / AmH)
Target Names
tgt
Target Protein Sequence
MEFRIDSIDG NARACTIKTE HSEIKTPIFM PVGTAAAVKS LDAIDMMEIL DAPIILGNTY
HLYLRPGDEI ISKLGGLHGF SGYKRSFLTD SGGFQAFSLG DNVKFTDEGI WFKSHIDGSK
HFFSPEKVID IEYNLNSDIM MVLDDLIALP NTKERIKKSI ERTTEWAYRS LVHHVNRGKK
NNLFAIIQGG TDFEFRRISA ESLVSLEYKG YTFDGFAIGG LSVGEENQLM YDTIEATTPY
MPKDKPRYLM GVGTPEDIIE AVERGVDMFD CVMPTRNARN GYLFTTFGTL RIKNAKYKLD
ESPIDPKCNC YTCRNFSRAY LNHLFKAKEL TYFRLASIHN LHYYLNFVKE IREAILEGRF
KEYKKEFYSL RAMNSLEI
Uniprot No.
Target Background
Function
Queuine tRNA-ribosyltransferase (tgt) catalyzes the replacement of guanine (G) with 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His, and -Tyr). This occurs via a double-displacement mechanism: the active site attacks C1' of nucleotide 34, removing guanine and forming a covalent enzyme-RNA intermediate. Then, the active site deprotonates PreQ1, facilitating its attack on the ribose C1' to create the product. After dissociation, further enzymatic reactions convert PreQ1 to queuine (Q), resulting in queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Database Links
KEGG: nam:NAMH_0482
STRING: 598659.NAMH_0482
Protein Families
Queuine tRNA-ribosyltransferase family
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