Recombinant Neisseria meningitidis serogroup C / serotype 2a Na (+)-translocating NADH-quinone reductase subunit E
Description
Genetic and Functional Context
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Complex Role: NqrE is a core subunit of the Na(+)-NQR complex, which comprises six subunits (A–F). This complex catalyzes:
The enzyme translocates 2–4 Na ions per NADH oxidized, depending on bacterial species .
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Metabolic Importance: In N. meningitidis, Na(+)-NQR is a primary respiratory sodium pump, enabling adaptation to host niches with varying sodium concentrations .
3.1. Evolutionary Adaptation in Hyperinvasive Strains
Genomic studies of pandemic N. meningitidis serogroup A (clonal complex 5) revealed horizontal acquisition of nqrE alleles from hyperinvasive serogroup W (cc11) strains. These introgressed alleles enhanced metabolic efficiency, likely contributing to epidemic spread :
| Introgression Event | Source Strain | Functional Impact |
|---|---|---|
| nqrE (Area G) | W:cc11 (1964) | Increased NADH oxidation kinetics in low-sodium environments |
This adaptation suggests Na(+)-NQR variants influence transmission fitness by optimizing energy metabolism under host-specific conditions .
3.2. Proteomic Regulation Under Stress
Proteomic profiling of N. meningitidis mutants lacking DNA-processing protein DprA showed downregulation of Na(+)-NQR subunits, including NqrE. This impaired respiratory chain activity and reduced resistance to oxidative stress, highlighting the enzyme’s role in bacterial survival during infection .
Product Specs
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Target Background
The NQR complex catalyzes the two-step reduction of ubiquinone-1 to ubiquinol, coupled with the translocation of Na+ ions from the cytoplasm to the periplasm. NqrA through NqrE are likely involved in the second step, converting ubisemiquinone to ubiquinol.
KEGG: nmc:NMC0506
