Recombinant Neisseria meningitidis serogroup C Transaldolase (tal)

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In Stock
Cat. No.
BT38555
Source
Yeast
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38555
Source
E.coli
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38555
Source
E.coli
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38555
Source
Baculovirus
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38555
Source
Mammalian cell
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Purity
>85% (SDS-PAGE)
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Description

Introduction to Transaldolase in Neisseria meningitidis

Transaldolase (Tal) is a key enzyme in the pentose phosphate pathway (PPP), critical for nucleotide biosynthesis and maintaining cellular redox balance. In Neisseria meningitidis serogroup C (NmC), Tal is encoded by the tal gene, which has been extensively studied as part of multilocus sequence typing (MLST) schemes to understand bacterial evolution and clonal complex classification . Recombinant Tal refers to the enzyme produced through genetic engineering, enabling detailed structural, functional, and immunological studies.

Genetic Characteristics of the tal Gene

The tal gene in N. meningitidis serogroup C is a housekeeping locus used in MLST. Key features include:

PropertyValueSource
Gene length (bp)492
GC content (%)11.0
Polymorphism (SNPs)15
Allelic diversity0.237

Notes:

  • The low GC content (11%) suggests horizontal gene transfer or recombination events in its evolutionary history .

  • Allelic diversity (0.237) indicates moderate sequence variation among strains, critical for phylogenetic analyses .

Role in MLST and Phylogenetic Studies

The tal gene is part of an extended MLST panel (20 loci) used to resolve clonal relationships among meningococcal strains :

  • Clonal complex classification: Tal helps differentiate hyperinvasive lineages (e.g., cc11, cc32) from less pathogenic strains.

  • Recombination impact: Despite frequent recombination in N. meningitidis, tal retains sufficient stability for genealogical inference .

  • Comparative utility: Analysis of 20 loci, including tal, improved resolution of clonal complexes compared to the standard 7-locus MLST .

Key Studies:

  1. Genealogical Typing:

    • A study analyzing 93 meningococcal isolates using 20 loci, including tal, identified six major hyperinvasive lineages .

    • tal polymorphisms supported clustering of serogroup C strains into clonal complexes (e.g., cc11) .

  2. Recombination Dynamics:

    • The tal locus exhibited lower GC content compared to other housekeeping genes (e.g., glnA: 13.0%), suggesting distinct evolutionary pressures .

Data Table: Comparison of tal with Other MLST Loci

GeneLength (bp)GC (%)Polymorphism (SNPs)
tal49211.015
glnA47113.011
aroE4989.027

Future Directions

  • Structural studies: Recombinant Tal production could enable crystallography to elucidate substrate-binding mechanisms.

  • Vaccine development: While polysaccharide/protein conjugate vaccines target capsular antigens (e.g., MenC-TT) , housekeeping enzymes like Tal remain underexplored as secondary targets.

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. Request dry ice in advance for an additional fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize its development.
Synonyms
tal; NMCC_1791; Transaldolase; EC 2.2.1.2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-351
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Neisseria meningitidis serogroup C (strain 053442)
Target Names
tal
Target Protein Sequence
MTILSDVKAL GQQIWLDNLS RSLVQSGELA QMLKQGVCGV TSNPAIFQKA FAGDALYADE VAALKQQDLS PKQRYETMAV ADVRAACGIC LAEHESTGGK TGFVSLEVSP ELSKDAQGTV EEARRLYAAI GCKNAMIKVP ATDAGIDALE TLVSDGISVN LTLLFSRVQT LKAYAAYARG IAKRLAAGQS VAHIHVVASF FISRVDGALD TTLPDHLKGK IAIALAKAAY QDWEQYFTTP EFAALEAQGA NRVQLLWAST GVKNPAYPDT LYVDNLIGAH TVNTVPDATL KAFIDHGTAK ATLTEGVEEA QAQLAETAAL GIDVETLANR LQEDGLKQFE EAFEKLLAPL V
Uniprot No.
A9M2N9

Target Background

Function
Transaldolase is essential for metabolite balance in the pentose-phosphate pathway.
Database Links

KEGG: nmn:NMCC_1791

Protein Families
Transaldolase family, Type 2 subfamily
Subcellular Location
Cytoplasm.

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