Recombinant Neosartorya fischeri Mitochondrial thiamine pyrophosphate carrier 1 (tpc1)
Description
Molecular Characterization
Recombinant tpc1 is a 317-amino acid protein (UniProt ID: A1DI57) expressed in E. coli with an N-terminal His-tag for purification . Key structural and biochemical properties include:
The protein belongs to the mitochondrial carrier family (MCF), characterized by six transmembrane domains critical for substrate translocation .
Biological Function
tpc1 facilitates the transport of ThPP—a coenzyme essential for acetyl-CoA synthesis and carbohydrate metabolism—across the mitochondrial inner membrane. Key functional insights include:
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Substrate Specificity: Demonstrates high affinity for ThPP but also transports pyrophosphate and nucleotides (ADP/ATP) in exchange .
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Mechanism: Operates via an electroneutral antiport system, exchanging ThPP for intramitochondrial ATP or ADP .
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Genetic Complementation: Heterologous expression of tpc1 homologs in Saccharomyces cerevisiae TPC1-null mutants restores growth on fermentable carbon sources, confirming functional conservation .
Biochemical Reconstitution Studies
Reconstitution of purified tpc1 into liposomes enables kinetic analysis of ThPP transport. Key parameters derived from homologous systems include:
| Parameter | Value | Source Organism |
|---|---|---|
| (ThPP) | 12.6 ± 1.8 µM | Drosophila melanogaster |
| 68 ± 5 nmol/min/mg protein | Drosophila melanogaster | |
| pH Optimum | 7.0–7.5 | Saccharomyces cerevisiae |
These data suggest conserved transport kinetics across fungal and metazoan homologs .
Industrial and Biomedical Relevance
While direct therapeutic applications of tpc1 remain unexplored, related Neosartorya fischeri proteins (e.g., NFAP antifungal peptides) exhibit biofungicidal activity against phytopathogens and Candida auris biofilms . tpc1’s role in mitochondrial metabolism positions it as a potential target for metabolic engineering or antifungal adjunct therapies.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you require a specific format, please indicate your preference in the order notes. We will prepare the product according to your request.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. For the lyophilized form, the shelf life is 12 months at -20°C/-80°C.
Target Background
KEGG: nfi:NFIA_090220
