Recombinant Neosartorya fumigata GPI mannosyltransferase 3 (gpi10)
Description
Functional Role in Fungal Biology
GPI mannosyltransferase 3 catalyzes the addition of mannose residues during GPI anchor assembly, which anchors proteins to the cell membrane. Key findings include:
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Cell Wall Integrity: GPI-anchored proteins are critical for fungal cell wall structure. Disruption of gpi10 homologs in Aspergillus fumigatus leads to cell lysis, abnormal hyphal growth, and defective conidiation .
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Virulence: gpi10 deletion mutants in A. fumigatus exhibit attenuated virulence in immunocompromised mice, highlighting its role in pathogenicity .
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Immune Evasion: GPI-anchored surface proteins like CcpA in A. fumigatus modulate host immune responses by altering conidial surface recognition .
Recombinant Production and Applications
Expression Systems
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Hosts: Flexible production in E. coli, yeast, or mammalian cells .
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Yield: High-purity (>85%) protein suitable for structural studies and enzymatic assays .
Research Applications
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Enzymatic Studies: Used to characterize mannosyltransferase activity in GPI biosynthesis .
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Drug Development: Target for antifungals disrupting GPI anchor synthesis, a pathway absent in humans .
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Comparative Genomics: Homology studies across fungi (e.g., Aspergillus oryzae, Saccharomyces cerevisiae) reveal evolutionary conservation .
Related Recombinant Proteins
| Species | Gene ID | Protein Length | Host System |
|---|---|---|---|
| Gibberella zeae | FGSG_05545 | Partial | E. coli/Yeast |
| Candida glabrata | CAGL0F07843g | Partial | Mammalian Cells |
| Saccharomyces cerevisiae | GPI10 | Partial | Baculovirus |
Data sourced from MyBioSource and Creative BioMart .
Research Implications
Studies on recombinant GPI10 have clarified its role in fungal pathogenesis and cell wall dynamics. For example:
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will fulfill your request.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: afm:AFUA_4G09130
STRING: 5085.CADAFUBP00006447
