Recombinant Neosartorya fumigata High osmolarity signaling protein sho1 (sho1)
Description
Definition and Biological Context
Recombinant Sho1 is a full-length, His-tagged protein expressed in E. coli and derived from Neosartorya fumigata (the teleomorph of Aspergillus fumigatus) . It is a transmembrane sensor protein involved in the High Osmolarity Glycerol (HOG) mitogen-activated protein kinase (MAPK) pathway, which enables fungi to adapt to osmotic and oxidative stress . The protein features four transmembrane domains and a C-terminal Src Homology 3 (SH3) domain, facilitating interactions with downstream signaling components like Pbs2 MAPK .
Biochemical and Biophysical Properties
Key characteristics of recombinant Sho1 include:
Role in Stress Adaptation
Sho1 regulates fungal responses to environmental stressors:
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Oxidative Stress: A. fumigatus Δsho1 mutants show hypersensitivity to hydrogen peroxide (2.5 mM) and menadione (15 μM), indicating impaired redox homeostasis .
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Morphogenesis: Deletion of sho1 disrupts hyphal elongation, phialide formation, and conidiation, leading to hyperbranched, stunted hyphae .
Phenotypic Analysis of Δsho1 Mutants
| Parameter | Wild-Type | Δsho1 Mutant | Complemented Strain |
|---|---|---|---|
| Radial Growth Rate | Normal | Reduced by 40% | Restored |
| Germination Delay | None | 2–4 hours | Restored |
| Hyphal Morphology | Regular | Hyperbranched | Restored |
| Oxidative Stress Sensitivity | Low | High | Restored |
Source: In vitro studies using A. fumigatus models .
Pathogenic Implications
Despite its role in stress adaptation, Δsho1 mutants retained full virulence in immunocompromised murine models, suggesting compensatory pathways in A. fumigatus pathogenesis .
Applications and Relevance
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Biotechnological Use: Recombinant Sho1 serves as a tool for studying fungal stress signaling and HOG pathway mechanics .
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Therapeutic Targets: Sho1 homologs in pathogenic fungi (e.g., Candida albicans) are explored for antifungal drug development .
Homology and Cross-Species Conservation
Sho1 is conserved across fungi, with 71–83% sequence identity to Saccharomyces cerevisiae and Aspergillus nidulans homologs . In N. fumigata, its function aligns with A. fumigatus, underscoring evolutionary conservation in stress response mechanisms .
Limitations and Future Directions
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific requirements for the format, please indicate them in your order remarks, and we will prepare the product according to your needs.
Note: All proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please notify us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
