Recombinant Neurospora crassa Autophagy-related protein 3 (atg-3)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify any format preferences in your order notes for customized preparation.
Note: While we will prioritize shipping the format currently in stock, please specify any format preferences in your order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional charges apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested and agreed upon in advance. Additional charges apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and can serve as a guideline.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
apg-3; atg3; NCU01955Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme atg3
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-352
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Target Names
apg-3
Target Protein Sequence
MNFLRSTAAT LLDKYTPVSH TSTFRNTGQI TPEEFVAAGD YLTFKFPSWS WADADSPSKR
LPFLPPGKQF LVTRHVPCHR RLNDDFAGDA GHEEALVEGN KGGADDDGWL RTGSMTSSQP
LRVREVRTVD DAGNVGDREV VDEDDIPDME DDDDDEAIIR AEGDNSNRQD NISTGKRTYT
LYITYANAYK CPRMYMSGYL SNGQPLPPHL MMEDIVGDYK DKTVTLEDFP FFSHSVKMAS
VHPCRHASVM KTLLDRADAA LKLRREKMKA GQGSGSEQGM EGLVDEINKL DVSGAHANAV
EAAPGEDAEW EEVPHDVADQ EVAIRVDQYL VVFLKFIASV TPGIEHDFTM GV
Uniprot No.
Target Background
Function
E2 conjugating enzyme crucial for cytoplasm-to-vacuole transport (Cvt) and autophagy. It is essential for selective autophagic degradation of the nucleus (nucleophagy) and mitophagy, regulating mitochondrial quantity and quality by maintaining a basal mitochondrial level to meet cellular energy demands and prevent excessive ROS production. It facilitates the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Glycine of apg-6/atg8. The atg12-apg-4/atg5 conjugate functions as an E3, promoting apg-6/atg8 transfer from apg-3/atg3 to phosphatidylethanolamine (PE), a process required for apg-6/atg8 membrane association. The resulting apg-6/atg8-phosphatidylethanolamine conjugate is vital for autophagy and Cvt, mediating membrane tethering and hemifusion, thereby expanding the autophagosomal membrane during autophagy.
Database Links
KEGG: ncr:NCU01955
Protein Families
ATG3 family
Subcellular Location
Cytoplasm.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
