Recombinant Neurospora crassa Crossover junction endonuclease mus-81 (mus-81), partial
Description
General Information
The Neurospora crassa Mus-81 is a crossover junction endonuclease that interacts with Eme-1 to form a DNA structure-specific endonuclease . This endonuclease demonstrates a substrate preference for branched DNA structures that have a 5'-end at the branch nick .
Role in Meiosis and DNA Repair
In Saccharomyces cerevisiae, the Mus81/Mms4 endonuclease promotes a distinct subset of crossovers during meiosis, acting independently of double-Holliday junction resolution . A study has indicated that Neurospora crassa uses symmetric heteroduplexes during meiotic recombination, which arise from Holliday junction migration .
The LSH/DDM1 homolog MUS-30 is required to prevent DNA damage arising from toxic base excision repair intermediates . MUS-30-deficient cells are hypersensitive to DNA damaging agents .
Relationship with MUS-51
mus-51 RIP70 is an allele used for high-efficiency targeted integration of transgenes into the genome of Neurospora crassa .
MUS81 in Telomere Biology
Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells .
Further Research
Product Specs
Target Background
This protein interacts with eme-1 to form a DNA structure-specific endonuclease. It preferentially targets branched DNA structures with a 5'-end at the branch nick, including 3'-flap structures, D-loops, replication forks, and nicked Holliday junctions. It may be crucial in mitosis for processing stalled or collapsed replication fork intermediates, and in meiosis for repairing meiosis-specific double-strand breaks following single-end invasion (SEI).
KEGG: ncr:NCU07457
