Recombinant Neurospora crassa rRNA-processing protein efg-1 (efg-1)

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Description

Introduction to Recombinant Neurospora crassa rRNA-processing protein efg-1

Recombinant Neurospora crassa rRNA-processing protein efg-1 is a recombinant form of the protein efg-1, which plays a crucial role in ribosomal RNA (rRNA) processing. This protein is essential for the proper assembly and maturation of ribosomal subunits, particularly in fungi like Neurospora crassa. The recombinant version of this protein is produced in various expression systems, including yeast, E. coli, and mammalian cells, allowing for its study and application in molecular biology and biotechnology.

Production and Sources of Recombinant efg-1

Recombinant Neurospora crassa efg-1 is available from several sources, including commercial suppliers who produce it in different expression systems:

Expression SystemDescription
YeastHigh purity, commonly used for protein production due to its eukaryotic nature .
E. coliOften used for large-scale production due to its rapid growth rate and cost-effectiveness .
BaculovirusUtilizes insect cells for expression, providing a more complex post-translational modification environment .
Mammalian cellsOffers the most complex post-translational modifications, useful for proteins requiring specific modifications .

Future Directions and Potential Applications

The recombinant Neurospora crassa efg-1 protein offers opportunities for further research into rRNA processing mechanisms and ribosome assembly. Potential applications include:

  • Biotechnology: Understanding efg-1's role could inform strategies for improving protein production in biotechnological systems.

  • Basic Research: Studying efg-1 in Neurospora crassa could provide insights into conserved mechanisms of rRNA processing across eukaryotes.

Product Specs

Form
Lyophilized powder

Note: While we will prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.

Lead Time
Delivery times vary depending on the purchasing method and location. Please contact your local distributor for precise delivery estimates.

Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing.

Tag type is determined during the production process. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms
efg1; NCU00092rRNA-processing protein efg1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-269
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Target Names
efg1
Target Protein Sequence
MGKRSYEEAE GSNADGQEQN QAQKRNRNPH NRFNPKNIAK RQKTTQKIND GNLSQIKKRV RAIERLLEHK NEKLPANVRN DLERELQAHK QRIVDESDRK LRSKMISKYH MVRFFERKKA MRLAKQLMKQ LDETKDEAEI ARLKADLHIA EVDLDYALYH PHMETYISLY PKPKDEAAEK DEKSSAAHHL HSSRPPMWTT IEKARQEGKS ALEKIRDRRP ERDFSKPTSK KTAKKSSSQS EERPAKGKFG KTEEESANES DDGGFFEED
Uniprot No.

Target Background

Function

Involved in rRNA processing.

Database Links

KEGG: ncr:NCU00092

Protein Families
EFG1 family
Subcellular Location
Nucleus, nucleolus.

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