Recombinant Neurospora crassa SWR1-complex protein 4 (swc-4), partial
Description
Key Features of SWC4 Homologs:
Functional Role in Chromatin Remodeling
SWC4 contributes to SWR1-C’s ATP-dependent H2A.Z deposition, which modulates nucleosome stability and transcriptional accessibility. In Neurospora:
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Interactions with NuA4 Complex: SWR1-C collaborates with the NuA4 histone acetyltransferase complex (via BRD-8) to regulate circadian clock genes like frq. Loss of BRD-8 disrupts H4 acetylation and RNA Pol II recruitment, suggesting SWR1-C’s indirect role in transcriptional elongation .
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DNA Repair and Genome Stability: SWR1-C subunits in plants and yeast facilitate double-strand break repair via homologous recombination . Neurospora SWC-4 may similarly stabilize chromatin during replication stress.
Recombinant Production and Applications
While no direct studies on Neurospora SWC-4 exist, recombinant methods for related proteins provide a template:
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Expression System: Likely produced in E. coli with affinity tags (e.g., His-tag) for purification, as seen for Neurospora CBP-4 .
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Functional Assays: Recombinant SWC-4 could be used to:
Research Gaps and Future Directions
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Structural Characterization: Cryo-EM or X-ray crystallography of Neurospora SWR1-C would clarify SWC-4’s role in complex assembly.
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Phenotypic Analysis: Knockout mutants of swc-4 in Neurospora could elucidate its impact on circadian rhythms, stress responses, and development .
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Cross-Species Comparisons: Functional complementation assays using yeast or plant SWC4 mutants could validate evolutionary conservation .
Implications for Biotechnology
Engineering SWC-4 variants might enhance fungal strains for industrial applications (e.g., secondary metabolite overproduction) or CRISPR-based chromatin editing .
Product Specs
Note: We will prioritize shipping the format we have in stock. However, if you have specific requirements for the format, please indicate them when placing your order. We will fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please notify us in advance. Additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: ncr:NCU04002
