Recombinant Nitrosomonas europaea Integration host factor subunit alpha (ihfA)
Description
Introduction to Recombinant Nitrosomonas europaea Integration Host Factor Subunit Alpha (ihfA)
Recombinant Nitrosomonas europaea Integration Host Factor subunit alpha (ihfA) is a recombinant protein derived from the bacterium Nitrosomonas europaea. This protein is part of the Integration Host Factor (IHF), which plays a crucial role in DNA binding and bending, influencing various cellular processes such as transcription, DNA replication, and recombination in bacteria.
Key Features of Recombinant ihfA
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Source Organism: Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
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Expression Systems: Available in multiple expression systems including Baculovirus, Yeast, E. coli, and Mammalian cells .
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Purity: High purity, typically above 85% as determined by SDS-PAGE .
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Sequence: The protein sequence starts with MALTKAELTD LLFENIGLNK REAKEIVECF YEEMRAALQN GDGVKLSGFG NFQLRTKPQR PGRNPKTGEE IPISARRVVT FHASQKLKSM VEANYRGESG TN .
Function and Role of Integration Host Factor
Integration Host Factor (IHF) is a heterodimeric protein composed of two subunits, alpha (ihfA) and beta (ihfB). It is essential for the regulation of gene expression by binding to specific DNA sequences and inducing sharp bends in the DNA. This bending can facilitate the assembly of transcriptional complexes or influence the accessibility of DNA to other proteins involved in transcription and replication.
Biological Significance
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Transcription Regulation: IHF influences the transcription of various genes by altering the DNA structure, thereby affecting the binding of RNA polymerase and other transcription factors.
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DNA Replication and Recombination: IHF can also play roles in DNA replication and recombination processes by facilitating the interaction between DNA and proteins involved in these processes.
Potential Applications
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Biotechnology: Recombinant proteins like ihfA can be used in biotechnological applications such as gene expression regulation and DNA manipulation.
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Basic Research: Useful for studying DNA-protein interactions and the mechanisms of transcriptional regulation in bacteria.
Product Specs
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The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: neu:NE0952
STRING: 228410.NE0952
