Recombinant Nitrosomonas europaea Protein-L-isoaspartate O-methyltransferase (pcm)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery timelines.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, offered as a guideline for customers.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
The tag type is determined during production. If a specific tag type is required, please inform us, and we will prioritize its development.
Synonyms
pcm; NE0949; Protein-L-isoaspartate O-methyltransferase; EC 2.1.1.77; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-222
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298)
Target Names
pcm
Target Protein Sequence
MSVRHSGIGM TSLRTRVRMV ERLREQGIKD EVVLAAMGFI PRHLFVEAAL ASRAYEDVAL
PINYGQTISS PWIVGRMTEL LRNGGSNSLR KILEIGTGCG YQTAVLAKIA SEVYSIERIG
PLLTRTRIRL RELGILNIHL KHADGMRGLP EAGLFDGIMM TAVIPEIPET LLEQLAMGGR
MVFPKGNRKQ YLCVIDHTTE GFVETILDEV MFVPILPGTI NR
Uniprot No.
Target Background
Function
Recombinant Nitrosomonas europaea Protein-L-isoaspartate O-methyltransferase (pcm)
This enzyme catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins, which arise from the spontaneous degradation of normal L-aspartyl and L-asparaginyl residues. It plays a crucial role in the repair and/or degradation of damaged proteins.
This enzyme catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins, which arise from the spontaneous degradation of normal L-aspartyl and L-asparaginyl residues. It plays a crucial role in the repair and/or degradation of damaged proteins.
Database Links
KEGG: neu:NE0949
STRING: 228410.NE0949
Protein Families
Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
Subcellular Location
Cytoplasm.
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