Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit D (nuoD)
Description
Introduction to Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit D (nuoD)
Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit D (nuoD) is a protein component of the NADH:quinone oxidoreductase complex I, also known as NDH-1, which plays a crucial role in the respiratory chain of bacteria. This enzyme complex is responsible for transferring electrons from NADH to ubiquinone, contributing to the generation of a proton gradient across the bacterial membrane, which is essential for ATP synthesis .
Structure and Function of nuoD
The nuoD subunit is part of the hydrophilic domain of the NDH-1 complex and is involved in forming a quinone binding cavity along with neighboring subunits . The recombinant nuoD protein from Nocardia farcinica is expressed in Escherichia coli and has a purity of over 85% as determined by SDS-PAGE . The sequence of the nuoD protein includes several conserved amino acid residues that are important for its function .
Characteristics of Recombinant nuoD
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Expression and Purity: The recombinant nuoD protein is expressed in E. coli with a purity of >85% .
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Sequence: The protein sequence includes several conserved regions crucial for its function, such as MKDTETRPGRHRAPEPAHPEQPDTTGDTVVTVSGQDWDTV .
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Storage Conditions: The shelf life of the liquid form is typically 6 months at -20°C/-80°C, while the lyophilized form can last up to 12 months under the same conditions .
Table 1: Characteristics of Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit D (nuoD)
| Characteristics | Description |
|---|---|
| Expression Host | Escherichia coli |
| Purity | >85% (SDS-PAGE) |
| Sequence | MKDTETRPGRHRAPEPAHPEQPDTTGDTVVTVSGQDWDTV... |
| Storage Conditions | Liquid: 6 months at -20°C/-80°C; Lyophilized: 12 months at -20°C/-80°C |
| Protein Length | Full-length protein (1-453 amino acids) |
Table 2: Comparison of NADH-quinone oxidoreductase Subunits
| Subunit | Function | Species |
|---|---|---|
| nuoD | Part of quinone binding cavity | Nocardia farcinica, Escherichia coli |
| nuoK | Electron transfer component | Nocardia farcinica |
| NuoCD | Proton channel in peripheral arm | Escherichia coli |
References Secreted protein NFA47630 from Nocardia farcinica IFM10152... Conserved Amino Acid Residues of the NuoD Segment Important for... Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit D (nuoD) ELISA Recombinant Nocardia farcinica NADH-quinone oxidoreductase subunit K(nuoK) MetaCyc NADH:quinone oxidoreductase I, peripheral arm StyA1 and StyA2B from Rhodococcus opacus 1CP...
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify any format requirements in your order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The specific tag type is determined during production. If you require a particular tag, please inform us, and we will prioritize its incorporation.
Target Background
KEGG: nfa:NFA_26640
STRING: 247156.nfa26640
