Recombinant Nocardia farcinica Sulfate adenylyltransferase subunit 2 (cysD)
Description
Introduction to Recombinant Nocardia farcinica Sulfate Adenylyltransferase Subunit 2 (cysD)
Recombinant Nocardia farcinica Sulfate adenylyltransferase subunit 2, commonly referred to by its gene name cysD, is a crucial enzyme involved in the sulfate assimilation pathway. This enzyme is part of the ATP sulfurylase complex, which catalyzes the conversion of sulfate into adenosine 5'-phosphosulfate (APS) and diphosphate. The recombinant form of this enzyme is produced in yeast and is available for research purposes, offering high purity and competitive pricing .
Biological Function of Sulfate Adenylyltransferase Subunit 2
Sulfate adenylyltransferase subunit 2, encoded by the cysD gene, plays a pivotal role in the metabolic pathway responsible for the assimilation of sulfate. It works in conjunction with another subunit, CysN, to form the ATP sulfurylase enzyme. This enzyme complex is essential for initiating the sulfate reduction pathway, which is vital for the synthesis of sulfur-containing amino acids and other sulfur compounds in bacteria .
Production and Characteristics
The recombinant Nocardia farcinica Sulfate adenylyltransferase subunit 2 is produced in yeast, ensuring high purity and stability. This recombinant protein may include N-terminal and C-terminal tags, which are determined based on factors such as tag-protein stability . The shelf life of the recombinant protein is influenced by storage conditions, buffer ingredients, and other environmental factors .
Research Findings and Applications
Research on sulfate adenylyltransferase subunit 2 has primarily focused on its enzymatic activity and role in bacterial metabolism. The enzyme's ability to catalyze the formation of APS is critical for subsequent steps in sulfate assimilation, including the reduction of APS to sulfite and eventually to sulfide, which is then incorporated into amino acids and other biomolecules .
Table 1: Key Features of Recombinant Nocardia farcinica Sulfate Adenylyltransferase Subunit 2
| Feature | Description |
|---|---|
| Production Host | Yeast |
| Purity | High |
| Function | Catalyzes ATP + sulfate → diphosphate + adenosine 5'-phosphosulfate |
| Tags | May include N-terminal and C-terminal tags |
| Storage | Shelf life dependent on storage conditions |
Genomic Context and Related Pathways
Nocardia farcinica, the bacterium from which this enzyme is derived, has a complex genome with various metabolic pathways. The genomic sequence of N. farcinica reveals a high metabolic potential, including numerous oxygenases and other enzymes involved in secondary metabolism . The presence of sulfate adenylyltransferase subunit 2 highlights the bacterium's ability to utilize sulfate as a sulfur source, which is essential for its survival and metabolic processes.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes. We will fulfill requests as availability allows.
Note: All proteins are shipped with standard blue ice packs unless dry ice is specifically requested. Contact us in advance for dry ice shipping; additional charges will apply.
The tag type is determined during production. To request a specific tag, please inform us; we will prioritize fulfilling such requests.
