Recombinant Nostoc punctiforme Elongation factor G (fusA), partial
Description
Functional Role in Translation
Elongation factor G (EF-G/fusA) is essential for:
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Ribosome translocation: Moves tRNA and mRNA during the elongation phase of protein synthesis.
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GTP hydrolysis: Couples GTP hydrolysis to mechanical movement of ribosomal subunits .
In N. punctiforme, EF-G is hypothesized to support cellular processes critical for nitrogen fixation and stress adaptation, though direct evidence remains understudied.
Biotechnological Applications
Studies on fusA homologs in other bacteria provide insights into potential applications:
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Metabolic Engineering: Overexpression of fusA in Corynebacterium glutamicum enhanced L-isoleucine production by upregulating genes in branched-chain amino acid biosynthesis (e.g., ilvA, ilvBN) .
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Stress Response Modulation: In cyanobacteria like Anabaena, EF-G interacts with transcriptional regulators like LexA under γ-radiation stress, suggesting roles in oxidative stress management .
Research Implications
The partial recombinant fusA protein enables:
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Antibiotic Studies: EF-G is a target for fusidic acid, a steroid antibiotic inhibiting bacterial translation.
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Symbiosis Mechanisms: N. punctiforme forms symbioses with plants (e.g., Blasia pusilla), where motility and stress-response proteins like EF-G may facilitate host interactions .
Technical Considerations
Product Specs
Target Background
KEGG: npu:Npun_F3883
STRING: 63737.Npun_F3883
