Recombinant Nostoc sp. Allophycocyanin subunit alpha-B (apcD)
Shipped with Ice Packs 
In Stock 
Description
Functional Role
-
Energy Transfer:
-
Biosynthesis:
Heterologous Expression and Applications
-
Production in E. coli:
-
Applications:
| Expression System | Yield | Key Features | Source |
|---|---|---|---|
| E. coli (pGEX-2T) | 8–10 mg/L | GST-tagged apcD/ApcB heterodimer | |
| Synechococcus native | 5–7 mg/L | Native chromophorylated complex |
Interactions and Functional Partners
-
Core Phycobilisome Components:
-
Regulatory Proteins:
| Partner Protein | Function | Interaction Type | Source |
|---|---|---|---|
| ApcB | β subunit of AP-B heterodimer | Covalent binding | |
| apcE | Core linker/energy transfer mediator | Non-covalent binding | |
| cpcG | Rod-core linker | Structural stabilization |
Research Challenges and Advances
Product Specs
Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies depending on the purchase method and location. Consult your local distributors for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional fees apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
apcD; all3653Allophycocyanin subunit alpha-B
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-161
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Target Names
apcD
Target Protein Sequence
TVISQVILQ ADDELRYPSS GELKSISDFL QTGVQRTRIV ATLAENEKKI VQEATKQLWQ
KRPDFIAPGG NAYGERQRAL CIRDFGWYLR LITYGVLAGD IEPIEKIGII GVREMYNSLG
VPVPGMVEAI NSLKKASLDL LSSEDAAAAA PYFDYIIQAM S
Uniprot No.
Target Background
Function
Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. Allophycocyanin has a maximum absorption at approximately 654 nanometers.
Database Links
KEGG: ana:all3653
STRING: 103690.all3653
Protein Families
Phycobiliprotein family
Subcellular Location
Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side. Note=Forms the core of the phycobilisome.
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